相关实验视频

Updated: May 28, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.1K

TaqMan RT-qPCR用于tRNA半量化

Megumi Shigematsu1, Takuya Kawamura1, Yohei Kirino1

  • 1Computational Medicine Center, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, United States.

Methods in enzymology
|February 14, 2025
PubMed
概括

量化tRNA衍生小非编码RNAs (sncRNAs) 是由于序列异质性和与成熟tRNAs的共存而具有挑战性. 一种新的TaqMan RT-qPCR方法与适应器结合特异地量化了tRNA的一半,克服了这些限制.

关键词:
适配器的绑定方式量化 量化 量化 量化简短的非编码RNA (sncRNA)TaqMan RT-qPCR 在线服务提供商一半的tRNA半个

更多相关视频

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

664
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

24.4K

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
56.7K