沙门氏菌Typhimurium SL1344变种的分离和表征,它们对不同压力剂和食品加工技术的耐药性增加
S Guillén1, L Nadal1, N Halaihel2
1Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Instituto Agroalimentario de Aragón- IA2, Universidad de Zaragoza-CITA, 50013, Zaragoza, Spain.
Food microbiology
|February 14, 2025
概括
沙门氏菌Typhimurium变种对多种压力因素产生了稳定的耐药性,通常与hnr基因的突变和增加的RpoS活性有关. 这种耐药性影响了生长和毒性,但没有影响生物膜的形成.
科学领域:
- 微生物学 微生物学
- 细菌遗传学 细菌遗传学
- 压力反应应激反应
背景情况:
- 沙门氏菌 (Salmonella enterica serovar Typhimurium) 是一种重要的食物传播病原体.
- 了解抗压力机制对于食品安全和公共卫生至关重要.
- 获得耐药性可以影响细菌的健康和毒性.
研究的目的:
- 为了选择和描述沙门氏菌耐药变异Typhimurium SL1344.
- 研究获得的抗压能力的遗传和表型基础.
- 探索RpoS活动对抗压力和健身成本的作用.
主要方法:
- 通过反复暴露在酸,透应激,高水静压 (HHP) 和紫外线 (UV-C) 光中来选择耐药变体.
- 表型特征包括生长速度,毒性测定 (细胞粘附) 和生物膜形成.
- 全基因组测序 (WGS) 用于识别遗传突变.
- 对RpoS调节基因的基因表达分析.
主要成果:
- 孤立变体对多种压力因素表现出交叉抵抗.
- 获得的耐药性通常会在生长和受影响的细胞粘附方面产生适应性成本.
- 在5个变异中,在3个变异中发现了hnr基因的突变,导致RpoS活动增加.
- 增加的RpoS活动与较高的压力相关基因表达和较低的生长率相关.
结论:
- hnr基因和RpoS活动在沙门氏菌适应各种压力因素方面发挥着作用.
- 获得抗压能力可以导致增长和毒性的权衡.
- 在沙门氏菌菌株中,RpoS活性和应激抵抗之间可能没有直接的,普遍的关系.
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