相关实验视频

Updated: Jul 25, 2026

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

LINC00673与勒结合,调节牙周带干细胞中的骨质功能

Yang Yang^1,2, Peng Yang¹, Yuxing Zhang¹

¹Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University &Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, No.44-1 Wenhua Road West, Shandong, 250012, Jinan, China.

Journal of orthopaedic surgery and research

|February 14, 2025

概括

一个涉及LINC00673和microRNA-188-3p的调节网络影响牙周带干细胞中的骨形成. 这种相互作用会影响莱普的表达,为糖尿病相关的牙周炎提供潜在的治疗点.

科学领域:

分子生物学分子生物学
干细胞生物学干细胞生物学
牙周病学牙周病学

背景情况:

先进的糖化终产品 (AGEs) 创造了一个影响牙周带干细胞 (PDLSCs) 的微环境.
在AGE诱导的PDLSC骨质生成中,LINC00673和瘦素的作用需要进行研究.

研究的目的:

调查LINC00673,瘦素和PDLSC在AGE下的骨质生成之间的关联.
阐明涉及LINC00673,miR-188-3p和素在PDLSC骨质分化中的调节网络.

主要方法:

在骨质分化过程中对LINC00673表达的定量实时PCR (qRT-PCR).
性酸酶和阿里扎林红色S染色以评估骨质生成.
裸体小鼠背部测试用于骨再生和西部斑块用于骨质细胞标记物.

主要成果:

MiR-188-3p与LINC00673直接相互作用,促进骨质生成的分化.
LINC00673 作为 miR-188-3p 的竞争性内源性RNA,逆转了亲骨基效应.
一个LINC00673/miR-188-3p网络调节瘦素 (LEP) 表达,抑制Wnt通路并减少骨形成.

结论:

长非编码RNA (lncRNA) LINC00673和微RNA (miRNA) miR-188-3p之间的调节网络控制着PDLSC的骨质分化.
这种lncRNA-miRNA网络调节勒,影响骨的形成.
已确定的监管网络为糖尿病相关的牙周炎提供了潜在的治疗目标.

关键词:

先进的葡萄糖酶化最终产品.这里是 LINC00673 的位置.勒普丁是一种激素.长的非编码RNA是什么?骨质发生过程 (osteogenesis)

更多相关视频

Accessing the Cytotoxicity and Cell Response to Biomaterials

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Published on: February 17, 2023

相关概念视频

Osteoclasts in Bone Remodeling

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...