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使用气相色谱与高分辨率质谱相结合的干血点查测试的开发:在真实的死后样本中应用

Denis Dubois-Chabert¹, Estelle Flament¹, Guillaume Hoizey¹

¹LATLUMTOX Laboratory, Bron 69500, France.

Journal of analytical toxicology

|February 15, 2025

概括

这项研究开发了一种新的毒理学查方法,使用气体染色学高分辨率质谱法 (GC-HRMS) 对干血斑点 (DBS) 进行检测. 该方法为死后法医分析提供了高灵敏度和稳定性.

科学领域:

法医毒理学法医毒理学
分析化学分析化学
质谱测量质量谱测量

背景情况:

死后毒理学依赖于敏感和特定的分析方法.
干血斑 (DBS) 在样本体积,保存和处理方面具有优势.
气色谱-高分辨率质谱 (GC-HRMS) 提供高灵敏度和特异性.

研究的目的:

开发和验证使用GC-HRMS对死后DBS进行毒理学查分析.
评估化合物在DBS上的稳定性.
为了比较DBS-GC-HRMS方法与传统方法在真实的死后病例中的性能.

主要方法:

在提取和乙化后,使用GC-HRMS分析干血斑 (25μL).
对22种目标化合物确定了检测极限 (LOD) 和识别极限 (LOI).
化合物的稳定性在 -20°C,+4°C和+20°C下在15天内进行评估.
该方法应用于102个死后样本,并与LC-DAD-MS和GC-MS进行了比较.

主要成果:

LODs在<10.0和20.0 ng/mL之间,LOI处于治疗性或非中毒范围之内.
大多数化合物在DBS上表现出良好的稳定性,除了米达佐拉姆和特拉马多尔外.
DBS-GC-HRMS方法发现了81%的阳性病例,超过了传统的LC-DAD-MS (57%) 和GC-MS (60%).

结论:

开发的DBS-GC-HRMS方法是敏感的,有选择性的,适合进行死后毒理学查.
DBS为法医毒理学提供了可行的矩阵,在样品体积,保存和易用方面具有优势.
这种方法增强了死后调查中的毒理学发现,特别是在样本供应有限的情况下.

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