基于脂质和多变量分析,以确定细胞对pH值变化和缓冲组合的反应
Ricardo F S Pereira1,2, Carla C C R de Carvalho3,4
1iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal.
Marine biotechnology (New York, N.Y.)
|February 15, 2025
概括
货物缓冲器为海洋生物过程中传统的酸盐缓冲器提供了可行的替代方案. 像MES和EPPS这样的特定缓冲剂通过优化pH控制,显著增强了prodigiosin的产量.
科学领域:
- 生物技术是生物技术.
- 微生物生理学 微生物生理学
- 生物化学工程 生物化学工程
背景情况:
- 控制pH值在海洋生物过程中至关重要,但传统的缓冲物如酸盐会导致降水.
- 摇瓶中的有氧发酵通常缺乏活跃的pH控制,仅依赖于中等缓冲容量.
研究的目的:
- 在海洋生物工艺中评估Good's缓冲器作为酸盐缓冲器的替代品.
- 为了研究不同缓冲区和pH值对Serratia rubidaea. prodigiosin生产的影响.
- 了解细胞对不同缓冲组合的反应,特别是脂质组变化.
主要方法:
- 在不同的pH值下使用各种Good's缓冲剂 (MES,EPPS,HEPES,TRIS) 和酸盐缓冲剂发酵Serratia rubidaea.
- 脂组学分析以评估细胞膜组成.
- 统计多变量分析以解释脂质组数据并与生产指标相关联.
主要成果:
- 生物质生产率在不同缓冲区保持一致.
- 生素的产生受到缓冲器类型和pH值的显著影响,pH值为5.5的MES产生了最高的产量 (249.8 mg/L).
- 在pH 7.0的EPPS,HEPES和TRIS缓冲剂在海洋环境中被证明是酸盐缓冲剂的有效替代品.
- 细胞通过根据缓冲物种改变膜脂肪酸成分来表现出适应性.
结论:
- 货物缓冲器是酸盐缓冲器的有效替代品,可以增强代谢物生产.
- 缓冲区选择和pH优化是成功开发海洋生物工艺的关键因素.
- 细胞适应缓冲成分强调了考虑缓冲-微生物相互作用的重要性.
相关概念视频
01:28pH Regulation in Cells
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pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
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01:20Protein Buffers in Blood Plasma and Cells
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The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...
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02:19Buffer Effectiveness
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Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
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02:45Calculating pH Changes in a Buffer Solution
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A buffer can prevent a sudden drop or increase in the pH of a solution after the addition of a strong acid or base up to its buffering capacity; however, such addition of a strong acid or base does result in the slight pH change of the solution. The small pH change can be calculated by determining the resulting change in the concentration of buffer components, i.e., a weak acid and its conjugate base or vice versa. The concentrations obtained using these stoichiometric calculations can be used...
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