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低电极电位增强电流的产生由Geobacter sulfurreducens生物膜:一个高通量研究研究

David Hernández-Villamor¹, Peishuo Li², Musa Aydogan²

¹Center for Microbial Ecology and Technology (CMET), Ghent University, Frieda Saeysstraat 1, Gent, 9052, Belgium; Center for Advanced Process Technology for Urban Resource Recovery (CAPTURE), Frieda Saeysstraat 1, Gent 9052, Belgium.

Biosensors & bioelectronics

|February 15, 2025

概括

在低潜力下培养的Geobacter sulfurreducens生物膜会变小,但会表现出增强的电荷传输和更高的电流密度. 这表明在热力学限制条件下,电荷载体产量增加.

科学领域:

电微生物学电微生物学
微生物的电化学
生物能源学生物能源学

背景情况:

硫降低生物利用不同的细胞外电子转移 (EET) 途径,受电子受体潜力的影响.
缺乏对EET机制和限制潜力的影响的完整理解.

研究的目的:

调查各种潜能 ([-0.25到0]V与SHE) 对Geobacter sulfurreducens电活性生物膜 (EABs) 的影响.
在不同的热力学条件下描述代谢能量生成和EET机制.

主要方法:

使用定制的高吞吐量系统进行128次并行实验.
在三个阶段500小时内,Grew axenic G. sulfur减少了EAB.
进行了电化学表征,以分析生物膜性能.

主要成果:

在低于中点潜力 ([-0.18到-0.16] V) 的潜力下生长的EAB显示出较慢的增长,50%较小的生物膜大小,与0V的电流相比,极平原电流高出2.4倍.
较低的潜在增长导致了增强的电荷传输和显著更高的电荷载体度 ([1.6对0.4]mM_e).
电荷载体度与阳极平原电流线性相关;低电位氧化还原池占可存储电荷的50%-70%.

结论:

较低的电位会导致电荷载体在G. sulfurreducens EABs中的过度表达.
高通量方法对于推进电微生物学研究具有价值.
调查结果提供了在热力学上具有挑战性的条件下对ETT监管的见解.

关键词:

在CLSM中,使用的是CLSM.在EET路径上.电动生物膜是一种电动生物膜.电极阵列是一个电极阵列.这种生物是Geobacter sulfurreducensens.高通量的高通量.

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