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报告不足的动物传染病原体Streptococcus pasteurianus的基因组特征和抗菌耐药性及其与Streptococcus suis的共同殖民

Shuoyue Wang¹, Xinchun Li¹, Chenxu Zheng¹

¹MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210014, China; WOAH Reference Lab for Swine Streptococcosis, Nanjing 210014, China.

Veterinary microbiology

|February 15, 2025

概括

动物传染病原体菌 (Streptococcus pasteurianus) 显示出适应性和多药性耐药性,并发现了新的抗生素耐药性基因. 这项研究有助于管理这种未被认可的猪和人类威胁.

科学领域:

微生物学微生物学
基因组学就是基因组学.
抗微生物耐药性抗微生物耐药性

背景情况:

菌杆菌 (Streptococcus pasteurianus) 是一种机会性病原体,也是一个报告不足的动物性疾病威胁,通常与猪中的菌杆菌 (Streptococcus suis) 一起检测到.
关于S. pasteurianus的共同殖民动态,基因组特征和抗生素耐药性概况的理解有限.

研究的目的:

开发一种多重PCR (mPCR) 试验,用于检测猪桃体样本中的S. pasteurianus和S. suis.
调查S. pasteurianus. 的基因组特征和抗生素耐药性模式.

主要方法:

开发了一种多重PCR (mPCR) 试验,并应用于827个猪桃体样本.
进行全基因组分析以评估基因组适应性.
鉴定和描述了抗生素耐药性基因和移动遗传元素.

主要成果:

在81个样本中,mPCR测定检测到S. pasteurianus和S. suis.
确定了一个开放的泛基因组,表明了高度的适应性.
发现了21种不同的抗生素耐药性基因,包括S. pasteurianus中 mef(A), msr(D), optrA, lnu(G), spw, dfrF和fexA的新型鉴定. 菌株WUSP082因移动遗传元素上的15个耐药基因而表现出多药耐药性.
携带抗性基因的基因组岛屿和质粒与S. suis和Enterococcus faecium.的基因组岛屿和质粒具有很高的相似性.

结论:

S. pasteurianus具有可适应的基因组和显著的多药性耐药性,通常由移动遗传元素介导.
该研究在S. pasteurianus中发现了新的抗生素耐药性基因,突出了其作为动物传染病剂的潜力.
这些发现为动物和人类健康中S. pasteurianus感染的监测和管理提供了关键的见解.

关键词:

抗微生物耐药性抗微生物耐药性共同殖民化的共同殖民基因组特征基因组特征这种病原体是Streptococcus pasteurianus.这是一种Streptococcus suis.

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