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TaANK-TPR1通过控制NLR蛋白TaRPP13L1的基因表达和蛋白质活性来增强小麦对条纹生的抵抗力

Shuangyuan Guo¹, Feng Zhang¹, Xiaoya Du¹

¹College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling, Shaanxi 712100, China.

Developmental cell

|February 15, 2025

概括

研究人员确定了TaANK-TPR1,一种蛋白质可以增强小麦对Puccinia striiformis f. sp.的免疫力. 通过调节核酸结合部位,氨酸丰富的重复 (NLR) 蛋白质. 过度表达TaRPP13L1可以提高耐性,而不会影响农学特征.

科学领域:

植物免疫力植物免疫力
分子植物病原体相互作用
农作物科学农作物科学

背景情况:

核酸结合部位,富含白素的重复蛋白 (NLR) 对于植物对病原体的防御至关重要.
在Puccinia striiformis f. sp.期间NLRs的特定作用和调节在小麦中Triticum (Pst) 感染的原因尚不清楚.

研究的目的:

研究小麦中NLR蛋白对Pst感染的功能和调节机制.
为了确定参与小麦疾病耐药性的新型蛋白质.

主要方法:

识别和描述一个含有ankyrin (ANK) 重复和四基重复 (TPR) 的蛋白质,TaANK-TPR1.
对TaANK-TPR1与NLR蛋白TaRPP13L1.1.的相互作用进行分析.
研究TaANK-TPR1对TaRPP13L1转录和小麦耐药性的影响.

主要成果:

TaANK-TPR1积极调节小麦对Pst和NLR免疫反应的抵抗力.
TaANK-TPR1促进了TaRPP13L1的同质化和细胞死亡,增强了耐药性.
TaANK-TPR1与TaRPP13L1促进体中的TGACGT基因结合,激活其转录并响应素酸 (JA) 信号.
过度表达TaRPP13L1使其具有强大的抗性,而不会对农业特征产生负面影响.

结论:

TaANK-TPR1是小麦中NLR介导的免疫力对Pst.对抗的关键调节者.
TaANK-TPR1-TaRPP13L1相互作用为增强作物抗病能力提供了一种新的机制.
TaRPP13L1是改进小麦抗性基因的有希望的目标.

关键词:

在 ANK-TPRR 中.梅杰亚·梅杰亚是什么意思在NLR中,它是NLR.转录规则转录规则转录规则小麦 - 小麦Pst

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