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用于心肌细胞的光 in situ 杂交协议

Zehao Yao¹, Lina Bai², Yu Nie³

¹State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China; 4+4 Medical Doctor Program, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Journal of molecular and cellular cardiology

|February 15, 2025

概括

我们开发了 cardioFISH,这是一种用于测量心肌细胞核流动性的新方法. 这种技术克服了细胞的复杂性,为心脏研究提供了准确的性评估.

科学领域:

心血管生物学心血管生物学
细胞生物学细胞生物学
遗传学遗传学是一个

背景情况:

准确测量心肌细胞核是理解心脏发育,功能和疾病的必要条件.
目前的方法,如光 in situ 杂交 (FISH),由于细胞复杂性,在心肌细胞中面临挑战.

研究的目的:

提出一个详细的,心肌细胞特异的FISH (心脏FISH) 协议.
为了能够在心肌细胞中进行强大而准确的核性评估.

主要方法:

开发了一种定制的酶性消化策略,以改善核可访问性和保持细胞完整性.
尽量减少了来自萨尔科默的自发光.
介绍了一个3D核可视化框架,用于在大型心肌细胞核中全面分析信号.

主要成果:

心脏FISH 协议是一个为期两天的程序.
它适用于心肌细胞发育的各个阶段.
该方法提高了核可访问性,并最大限度地减少了自发光.

结论:

CardioFISH为推进研究心肌细胞核化提供了一个强大的工具.
本协议解决了传统FISH在心肌细胞中的局限性.
它为心脏研究提供了心肌细胞性的全面分析.

关键词:

一个心肌细胞.光在现场混合化中的光.排卵性排卵性排卵性

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相关概念视频

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...