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Updated: May 27, 2025

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揭示CRISPR-Cas9作为精确抗菌疗法的进展:系统性审查

Hannay Crystynah Almeida de Souza¹, Pedro Panzenhagen², Anamaria Mota Pereira Dos Santos³

¹Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, Brazil; Department of Biochemistry, Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, Brazil; Graduate Program in Biochemistry (PPGBq), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, RJ, Brazil.

Journal of global antimicrobial resistance

|February 15, 2025

概括

集群定期间隔的短时间Palindromic重复 (CRISPR) -Cas9显示了通过使细菌重新敏感来对抗抗菌素耐药性的承诺. 纳米技术的整合为临床应用挑战提供了潜在的解决方案.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
公共卫生公共卫生

背景情况:

抗菌素耐药性 (AMR) 是一个日益严重的全球健康危机.
耐药性病原体通过遗传变异性和横向基因转移传播,主要是通过等离子体.
抗菌药物耐药性损害了基本治疗的有效性.

研究的目的:

审查聚类正规间隔短Palindromic重复 (CRISPR) 技术和Cas9核酶在打击AMR方面的潜力.
探索CRISPR-Cas9对特定抗性基因和细菌家族的应用.

主要方法:

系统的文献审查遵循PRISMA指南.
在PubMed,Embase和Scopus数据库中进行的搜索截至2023年7月.
专注于针对肠道细菌,特别是大肠杆菌的耐药基因的研究.

主要成果:

CRISPR-Cas9主要针对β-乳酸盐耐药性 (bla基因) 和胆固醇耐药性 (mcr-1基因).
等离子体载体是主要的输送方法,成功地使细菌菌株重新敏感.
据报道,CRISPR-Cas9在重新敏感化方面的疗效在4.7%至100%之间.

结论:

克里斯普尔-Cas9显示出作为一种特定的抗菌剂的潜力.
纳米技术集成的交付策略显示出克服临床应用挑战的前景.
未来的应用可能会看到CRISPR-Cas9取代传统的广谱抗菌剂.

关键词:

细菌耐药性的细菌耐药性贝塔-乳糖酶是一种临床病原体临床病原体基因组编辑基因组编辑具有多种药物耐药性的药物.

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