通过生物信息学分析和实验验证验,识别牙周炎中与铁亡相关的基因
Kecong Zhou1, Huiwen Chen1, Jiachen Dong1
1Department of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, PR China.
Journal of stomatology, oral and maxillofacial surgery
|February 15, 2025
概括
牙周炎涉及铁,一个细胞死亡过程. 这项研究确定了与牙周炎相关的五个与铁亡相关的基因,为这种炎症性疾病提供了潜在的治疗点.
科学领域:
- 生物医学研究的研究.
- 细胞生物学 细胞生物学
- 口腔病理学 口腔病理学
背景情况:
- 牙周炎是一种慢性炎症性疾病,影响牙周组织.
- 铁,一种依赖于铁的细胞死亡途径,与炎症状况有关.
- 了解铁病在牙周炎中的作用对于开发新疗法至关重要.
研究的目的:
- 为了确定与牙周炎相关的与铁死相关的基因.
- 为了验证这些基因在牙周炎病原体中的相关性.
- 探索牙周炎的潜在治疗点.
主要方法:
- 在牙周炎数据集中对差异表达基因的分析和与铁病基因集的交叉.
- 功能丰富分析 (GO,KEGG) 和蛋白质与蛋白质相互作用 (PPI) 网络分析以确定枢纽基因.
- 在牙周炎组织和受脂聚糖化物 (LPS) 刺激的人类牙纤维细胞 (HGFs) 中核基因表达的验证,有或没有ferrostatin-1 (Fer-1).
主要成果:
- 在受牙周炎影响的牙组织中观察到铁.
- 确定了24个差异表达的铁灭基因 (FerDEGs).
- 确定了五个枢纽基因,在炎症组织和LPS刺激的HGF中表达显著更高.
- 在HGFs中,高调的PTGS2和IL6表达被Fer-1逆转.
结论:
- 五个与ferroptosis相关的基因被突出显示为牙周炎的潜在关键参与者.
- 这些基因代表了未来对牙周炎病原体的研究的有希望的目标.
- 向铁亡途径可能为牙周炎提供新的治疗策略.
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