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Updated: Jul 24, 2026

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
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一种多功能和高效的方法来隔离核从低输入的冷保存组织,用于单核转录组学

Cristopher Segovia1,2, Vincent Desrosiers3,4, Fatemeh Khadangi1,2

  • 1Centre de recherche du CHU de Québec - Université Laval, Axe Oncologie, 1401, 18e rue, Québec, Québec, G1J 1Z4, Canada.

Scientific reports
|February 15, 2025
PubMed
概括

这项研究介绍了一种优化的单核RNA测序协议,可对罕见的人类组织进行详细分析. 该方法有效地从最小的组织量中提取细胞核,这对于在有限的生物样本上推进疾病研究至关重要.

关键词:
癌症 癌症 癌症 癌症原子核的隔离方法核的分类 核的分类组织异质性 组织异质性组织同质化组织同质化.文字转录学 (Transcriptomics) 是一个学科.

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相关概念视频

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.