在性结肠炎中通过长链RNA测序调节基因表达和多化
Zhe Zhang1, Dan Li2, Shihang Zheng1
1The Second Department of Gastroenterology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, 110004, China.
BMC genomics
|February 15, 2025
概括
这项研究使用长时间读取的RNA测序来确定关键的基因表达差异和性结肠炎 (UC) 中的替代性多化 (APA) 位点变化. 这些发现为UC病原和潜在的治疗点提供了新的见解.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 性结肠炎 (UC) 是一种慢性肠道疾病,其免疫媒介致病因不明.
- 了解UC的分子基础对于改善诊断和治疗至关重要.
研究的目的:
- 用牛津纳米孔技术的长读RNA测序 (ONT-RNA-seq) 来区分UC患者和健康对照群之间的基因表达.
- 研究替代多基化 (APA) 部位选择在UC病变发生中的作用.
主要方法:
- 从UC患者和正常对照 (NC) 收集结肠组织样本.
- 进行了RNA提取和ONT-RNA-seq.
- 进行生物信息学分析,包括差异基因表达 (DEG),功能丰富,APA位点分析和miRNA/RNA结合蛋白 (RBP) 目标预测.
主要成果:
- 在UC患者中发现了多个基因的显著改变表达,包括较低水平的ACSF2,NPY,SLC26A3,BRINP3和PKLPP2,以及较高水平的CCL20,CCL21,CD55,IDO1,LCN2等.
- 功能丰富分析将DEG与免疫和炎症反应联系起来.
- APA位点选择与UC基因表达调节有关,鉴定的APA基因可能通过miRNA和RBP相互作用调节基因表达.
- 五个关键的APA基因 (CD38,NCALD,SMIM31,GPX7,SWAP70) 被强调,因为它们在UC中的潜在作用.
结论:
- ONT-RNA-seq为UC分子机制提供了新的见解.
- 这项研究强调了基因表达调节和APA部位选择在UC病变发生过程中的重要性.
相关概念视频
Regulation of Expression at Multiple Steps
864
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
864
Experimental RNAi
6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
Regulation of Expression Occurs at Multiple Steps
22.4K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.4K
RNA-seq
9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
MicroRNAs
21.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K


