基于尿蛋白质组学的分析确定CHI3L1作为免疫标记物和膀癌的潜在治疗标
Chenyang Wang1,2, Kunpeng Li1,2, Runchun Huang3
1Gansu Province Clinical Research Center for Urology, Lanzhou University Second Hospital, Lanzhou, China.
BMC cancer
|February 15, 2025
概括
酶3像1 (CHI3L1) 通过影响M2巨细胞和PI3K-Akt通路,促进膀癌的进展. 抑制CHI3L1可能为膀癌提供一种新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 膀癌 (BCa) 是一个重大的临床挑战,预后不佳.
- M2巨细胞越来越多地与促进癌症进展有关.
- 识别与M2巨细胞相关的BCa中的关键分子驱动因素对于治疗开发至关重要.
研究的目的:
- 为了研究与M2巨相关的膀癌 (BCa) 中的枢纽基因.
- 评估这些枢纽基因的预后意义.
- 探索潜在的监管机制和治疗目标.
主要方法:
- 利用公共数据集的生物信息学分析 (GEO,TCGA) 和尿液蛋白质组学.
- 使用机器学习 (LASSO,SVM) 来识别差异表达的基因.
- 在体外和体内验证的结果,包括细胞增殖,迁移,入侵和异种移植瘤模型的测试.
- 使用TIMER数据库,与M2巨细胞透相关联的枢纽基因表达.
主要成果:
- 基因酶3像1 (CHI3L1) 被确定为与BCa.中M2巨细胞相关的关键基因.
- 增加CHI3L1表达与晚期BCa阶段和不良预后相关.
- 抑制CHI3L1阻碍了BCa细胞的增殖,迁移和入侵,增强了gemcitabine的疗效.
- 降低CHI3L1调节抑制PI3K-Akt信号通路,并与瘤微环境中的M2巨细胞透有显著的关联.
结论:
- 通过PI3K-Akt信号通路,CHI3L1促进了膀癌的进展.
- CHI3L1与膀癌瘤微环境中的M2巨细胞透有关.
- CHI3L1代表了膀癌治疗的潜在治疗标.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
02:36Chromatin Immunoprecipitation- ChIP
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...