使用色谱可分离性的原型多式树脂的评估
Eric Denbaum1, Mario A Gutierrez Diaz2, Scott H Altern2
1Department of Biochemistry and Biophysics and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
这项研究引入了多式离子交换 (MMA) 和阴离子交换 (MMC) 树脂的快速选方法. 结合特定的MMA和MMC树脂可以显著提高染色体学中的蛋白质分离效率.
科学领域:
- 生物化学 生物化学
- 化学工程是化学工程的重要组成部分.
- 材料科学 材料科学 材料科学
背景情况:
- 染色树脂的开发对于蛋白质净化至关重要.
- 高通量选方法加速了新型分离材料的发现.
- 了解树脂与蛋白质的相互作用,有助于设计先进的色谱介质.
研究的目的:
- 为了快速选多式离子交换 (MMA) 和阴离子交换 (MMC) 树脂.
- 通过模型蛋白库,在各种pH条件下评估树脂性能.
- 为了确定最佳的树脂组合,以协同分离蛋白质.
主要方法:
- 采用了一种高通量并行批量吸附屏幕,并有连续的盐步骤.
- 计算的分离系数和模拟的列色谱,以确定化配置文件.
- 进行了聚类分析,以将树脂化学与蛋白质化模式相关联.
- 量化单树脂和双树脂可分离性得分.
主要成果:
- 确定了具有明显蛋白质结合和化特征的特定MMA和MMC树脂.
- 证明含guanidine的MMA树脂和基于phthalimide的MMC树脂具有协同的分离能力.
- 树脂的功能组,几何学和色谱性能之间建立了相关性.
- 量化了树脂对分离复杂蛋白质混合物的能力.
结论:
- 开发的高通量选方法可以快速发现下一代染色树脂.
- 特定的MMA和MMC树脂的组合可以提高蛋白质分离效率.
- 这项技术加快了对染色体学最佳配体和操作条件的识别.
更多相关视频
08:07A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products
Published on: May 22, 2019
09:08Separation of Aldehydes and Reactive Ketones from Mixtures Using a Bisulfite Extraction Protocol
Published on: April 2, 2018
相关概念视频
Chromatographic Methods: Terminology
Chromatographic Resolution
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
Racemic Mixtures and the Resolution of Enantiomers
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...
Principles Of Column Chromatography
