T盒转录因子在调节双阴性T细胞的免疫调节功能方面发挥着重要作用
Yongle Wu1, Xiaotong Han2, Longyang Zhou2
1Department of Gastroenterology, Beijing Chao-Yang Hospital, Capital Medical University, 100020, Beijing, China.
Biochemical and biophysical research communications
|February 16, 2025
概括
T-box转录因子T-bet对于调节双阴性T (DNT) 细胞功能至关重要,包括它们分泌IFN-γ和杀死单细胞的能力. 这一发现增强了对DNT细胞免疫调节机制的理解.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子机制的分子机制
背景情况:
- 双阴性T (DNT) 细胞是免疫平衡至关重要的关键免疫调节细胞.
- 控制DNT细胞功能的精确分子机制仍然不完全理解.
研究的目的:
- 阐明T盒转录因子 (T-bet) 在调节DNT细胞功能,特别是IFN-γ分泌和免疫杀伤能力方面的作用.
- 研究T-bet在DNT细胞中的直接转录标.
主要方法:
- 在天然DNT细胞和CD4+T细胞转化DNT (cDNT) 细胞中IFN-γ分泌的比较分析.
- 基因淘汰研究 (T-bet) 评估对DNT细胞功能的影响.
- 转录组测序和染色体免疫沉降测序 (ChIP-seq) 用于识别T-bet目标.
主要成果:
- 包括cDNT细胞在内的DNT细胞表现出高水平的IFN-γ分泌,并表达T-bet.
- T-bet淘汰赛显著降低了IFN-γ的分泌,并降低了cDNT细胞对单细胞的细胞毒性活性.
- ChIP-seq确定T-bet是DNT细胞中细胞毒性和激活基因 (例如Gzma,Gzmb,Prf1,Cd28) 的直接调节者.
结论:
- T-bet在调节DNT细胞的免疫调节功能,包括它们的细胞毒性和分泌能力方面发挥着关键作用.
- 这项研究扩大了对DNT细胞效应机制的理解.
- 这些发现为在基于免疫细胞的疗法中利用DNT细胞提供了理论基础.
相关概念视频
T Cell Types and Functions
897
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
897
T Cell Activation and Clonal Selection
629
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
629
TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
Master Transcription Regulators
6.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.8K
Transcription Factors
75.6K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.6K
General Transcription Factors
5.1K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.1K


