纳米颗粒诱导了质内皮泄漏,促进了albuminuria水平
Yingqi Wang1, Shen Zhao1, Nengyi Ni1
1School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an 271016, China.
NanoImpact
|February 16, 2025
概括
负电荷的金纳米粒子 (Au NPs) 可以诱导纳米材料诱导的内皮泄漏 (NanoEL),破坏膜过屏障. 这个过程激活了Notch信号,导致尿路蛋白质增加和脏损伤的可能性.
科学领域:
- 纳米毒理学研究
- 脏生理学 脏生理学
- 材料科学 材料科学 材料科学
背景情况:
- 纳米材料具有有益的特性,但在长期接触人类时会带来风险.
- 暴露可以破坏淋巴膜过屏障 (GFB),通过Notch1信号通路激活引起白膜尿.
- 减少的质内分泌体和减少的VE-cadherin有助于内皮泄漏.
研究的目的:
- 为了合成和表征20纳米负电荷的金纳米粒子 (Au NPs).
- 研究这些AuNP对内皮细胞紧密结合和屏障完整性的影响.
- 为了证实AU NP诱导的内皮泄漏在球模型中及其与Notch信号的关系.
主要方法:
- 合成20nm负电荷的金纳米粒子 (Au NPs).
- 与人类静脉内皮细胞 (HUVECs) 共同化Au NPs,以评估紧密结断裂.
- 使用质血管内皮细胞构建内皮泄漏模型,研究NanoEL和Notch信号.
主要成果:
- 合成的20纳米Au NPs被证实会破坏HUVECs的紧密结,导致内皮细胞泄漏.
- 在质内皮细胞模型中,20nm Au NPs诱导了纳米材料诱导的内皮泄漏 (NanoEL).
- 这种纳米EL过程涉及Notch信号激活,并导致尿蛋白增加.
结论:
- 20纳米负电荷的Au NPs通过破坏细胞-细胞结合,诱导内皮泄漏.
- Au NPs在质内皮细胞中触发了NanoEL,激活了Notch信号,导致了专尿.
- 这项研究增强了对生物系统中的纳米粒子相互作用及其潜在毒性的理解.
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