相关实验视频

Updated: May 27, 2025

Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness

Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness

Published on: March 18, 2022

矩阵金属蛋白酶加速细胞周边矩阵的分解,并破坏骨关节炎中机械传导

Jule Nieuwstraten¹, Rosa Riester¹, Ulf Krister Hofmann²

¹Laboratory of Cell Biology, Department of Orthopedic Surgery, University Hospital of Tübingen, Waldhörnlestraße 22, D-7207 Tübingen, Germany.

Acta biomaterialia

|February 16, 2025

概括

在骨关节炎中,矩阵金属蛋白酶 (MMP) 降解细胞周矩阵 (PCM) 破坏了状细胞机械感知. 这种损伤通过转移离子通道功能来改变细胞对机械应激的反应,从而影响软骨的健康.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
生物化学生物化学

背景情况:

周细胞基质 (PCM) 对状细胞功能和关节软骨中的机械感知至关重要.
骨关节炎 (OA) 涉及渐进的软骨退化,由矩阵金属蛋白酶 (MMPs) 造成的PCM损伤是早期的标志.
了解PCM在OA发病过程中的作用对于开发向疗法至关重要.

研究的目的:

调查PCM降解和冠状细胞机制信号之间的功能关系.
阐明MMP-2,MMP-3和MMP-7对PCM结构和状细胞机械传导的影响.
为了识别PCM损伤后离子通道参与机械传导的变化.

主要方法:

用MMP-2,MMP-3和MMP-7化人类冠状体.
对PCM结构变化的组织学评估 (,VI型原).
原子力显微镜 (AFM) 用于测量细胞弹性和机械传导 (Ca2+过渡物).

主要成果:

MMP治疗导致了显著的代谢性PCM降解,改变了原VI和perlecan结构和chondron生物力学.
在对机械刺激的反应中,MMP诱导的PCM损伤减少了子Ca2+过渡物.
在MMP治疗后,机械转导从TRPV4转移到PIEZO通道,特别是在类似于OA的条件下.

结论:

由MMP介导的PCM降解严重损害了状细胞的机械感知,并改变了机械传导通路.
PCM的结构完整性对于正常的冠状细胞功能和对机械线索的反应至关重要.
针对MMP活动和维护PCM完整性可能为OA提供治疗策略.

关键词:

合金子 (chondrons) 是一种类型.机械生物学机械生物学信号传递机制信号传递机制骨关节炎是一种骨关节炎.第1章一块东西在PIEZO2PIEZO2 在 TRPV4 中.

更多相关视频

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Published on: January 22, 2019

Application of Atomic Force Microscopy to Detect Early Osteoarthritis

Application of Atomic Force Microscopy to Detect Early Osteoarthritis

Published on: May 24, 2020

相关概念视频

Role of Matrix Metalloproteases in Degradation of ECM

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...

The Extracellular Matrix

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...

Osteoclasts in Bone Remodeling

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...

Extracellular Matrix

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

The Bone Matrix

The Bone Matrix

Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in...