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在低纤维饮食中,硫糖溶解维持了Eubacterium rectale的存在

Mahima Sharma¹, Nicholas Pudlo², Michael A Järvå³

¹York Structural Biology Laboratory, Department of Chemistry, University of York, York, United Kingdom.

The Journal of biological chemistry

|February 16, 2025

概括

欧细菌 rectale利用植物糖,如硫基诺 (SQ) 的生长. 补充SQ可以在饮食变化期间保持这种重要的Butyrate产生的肠道细菌,这表明其益生菌的潜力.

科学领域:

微生物学微生物学
生物化学生物化学
营养科学营养科学

背景情况:

细菌 (Bacillota) 产生短链脂肪酸 (SCFA),对肠道健康至关重要.
作为Firmicutes的一员的Eubacterium rectale可以代谢植物衍生糖硫基诺 (SQ).
SCFA,特别是酸盐,在人类肠道和疾病状态中起着至关重要的作用.

研究的目的:

调查Eubacterium rectale代谢硫诺维索基甘 (SQGro) 的能力.
阐明SQ代谢途径中关键酶的结构和功能作用.
评估SQ作为益生菌在模拟肠道环境中支持E. rectale种群的潜力.

主要方法:

在SQGro.上进行生化分析以确认E. rectale生长.
对E. rectale sulfoquinovosidase (SftG) 和SftX (DUF4867) 的3D结构确定.
对保护的蛋白质域进行序列相似性网络的分析.
在无菌小鼠中使用合成微型人类微生物组来测试SQ补充剂的效果.

主要成果:

E. rectale可以在SQ和SQGro上生长.
在GH31SQases中保存了SftG的3D结构,并且SftX (DUF4867) 的功能被上下文化.
在小鼠模型中,SQ补充在转向低纤维,高脂肪饮食期间拯救了E. rectale种群.

结论:

E. rectale 对植物衍生硫糖具有独特的代谢能力.
SQ和SQGro是潜在的益生菌,可以维持有益的酸盐产生细菌,如E. rectale. rectale.
了解这些途径可以为调节肠道微生物群组成和功能的策略提供信息.

关键词:

生物信息学是一种生物信息学.碳水化合物代谢的代谢葡萄糖糖酸酶的使用方法微生物组是一个微生物组.益生菌益生菌益生菌结构生物学结构生物学

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