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相关概念视频

Protein Networks02:26

Protein Networks

3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Protein-protein Interfaces02:04

Protein-protein Interfaces

12.4K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

7.1K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
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相关实验视频

Updated: May 27, 2025

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
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Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

Published on: May 18, 2020

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码头:资源扩展到蛋白质-RNA相互作用体

Keeley W Collins1, Matthew M Copeland1, Petras J Kundrotas1

  • 1Computational Biology Program, The University of Kansas, Lawrence, KS 66045, United States.

Journal of molecular biology
|February 16, 2025
PubMed
概括

计算建模有助于理解蛋白质-RNA相互作用,这对细胞过程和疾病研究至关重要. 现在Dockground数据库包括蛋白质-RNA复合体来支持这项研究.

关键词:
分子数据库,分子数据库.分子识别分子识别蛋白质对接的对接方式蛋白质-RNA复合体中的蛋白质.结构建模结构建模

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Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
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Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

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Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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Novel RNA-Binding Proteins Isolation by the RaPID Methodology

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相关实验视频

Last Updated: May 27, 2025

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
08:46

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

Published on: May 18, 2020

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Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

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Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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Novel RNA-Binding Proteins Isolation by the RaPID Methodology

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科学领域:

  • 结构生物学 结构生物学
  • 计算生物学 计算生物学
  • 分子生物学分子生物学

背景情况:

  • RNA调节细胞过程并结合蛋白质;调节失调与癌症等疾病有关.
  • 从结构上描述蛋白质-RNA相互作用对于理解分子机制至关重要.
  • RNA的灵活性使实验结构的确定变得复杂,导致可用的数据稀缺.

研究的目的:

  • 扩展Dockground资源,用于蛋白质-RNA相互作用的计算建模.
  • 为实验确定蛋白质-RNA复合体提供一个全面的数据库.
  • 促进蛋白质-RNA系统的基于结构的建模技术的开发和基准测试.

主要方法:

  • 扩展了Dockground数据库,包括蛋白质-RNA相互作用.
  • 在Dockground网站 (https://dockground.compbio.ku.edu/) 整合了新的功能.
  • 根据各种标准启用搜索,包括在不同的相似度值处删除冗余.
  • 实施了每周更新,使用来自蛋白质数据库的新结构.

主要成果:

  • 现在Dockground资源包含一个专门的数据集,用于蛋白质-RNA相互作用.
  • 更新后的网站提供了增强的搜索和数据管理功能.
  • 数据库定期更新,确保访问最新的结构信息.
  • 通过提供有价值的资源,促进了计算建模工作.

结论:

  • 扩展的Dockground数据库显著增强了蛋白质-RNA相互作用的研究.
  • 本资源支持用于基于结构的建模的计算方法的开发.
  • 它有助于研究人员了解涉及RNA结合蛋白的细胞过程和疾病的结构基础.