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Updated: May 8, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
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在Rhodotorula toruloides中发现,表征和应用染色体整合部位用于稳定的异质基因表达

Hao Xu1, Longyuan Shi1, Aashutosh Girish Boob1

  • 1Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States.

Metabolic engineering
|February 16, 2025
PubMed
概括

研究人员在化学生产酵母Rhodotorula toruloides中确定并验证了新的基因组整合部位. 这一突破使精确的基因工程成为可能,并加强了新生物技术的发展.

关键词:
生物信息学是一种生物信息学.克里斯普尔-Cas9是什么意思代谢工程是代谢工程.多复制基因整合多复制基因整合罗多托鲁拉 (Rhodotorula toruloides) 是一种多类动物.有针对性的DNA整合.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...