Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Metal-free synthesis of Pyrrolo[2,1-a]isoquinolines for type I photodynamic therapy and thermally activated delayed fluorescence materials.

Communications chemistry·2026
Same author

Prevalence and Epidemiological Characteristics of <i>Mycoplasma synoviae</i> Infection in Chickens in Mainland China.

Animals : an open access journal from MDPI·2026
Same author

IL-4 in Alzheimer's Disease-Mechanisms and Therapeutic Potential.

Molecular neurobiology·2026
Same author

Association of asthma and COPD with pertussis risk: A systematic review and meta-analysis.

Respiratory medicine·2026
Same author

Molecular mechanisms of plant freezing tolerance: from cold signal perception to adaptive responses.

Frontiers in plant science·2026
Same author

Effects of Neural Correlates of Food-Specific Intentional Inhibition in Predicting Body Fat Loss for Overweight and Normal-Weight Young Adults: The Mediation of Restrained Eating.

Nutrients·2026

相关实验视频

Updated: May 27, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.0K

PbImpute:在单细胞RNA测序数据中精确的零歧视和平衡的归算.

Yi Zhang1,2, Yin Wang1,2, Xinyuan Liu1,2

  • 1School of Computer Science and Engineering, Guilin University of Technology, 12 Jiangan Road, Qixing District, Guilin 541004, China.

Journal of chemical information and modeling
|February 17, 2025
PubMed
概括

PbImpute通过平衡脱落恢复和生物零保存,准确地归因单细胞RNA测序 (scRNA-seq) 数据. 这种方法通过提高数据准确性来增强细胞异质性分析和疾病研究.

更多相关视频

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

7.6K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.4K

相关实验视频

Last Updated: May 27, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.0K
Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

7.6K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.4K

科学领域:

  • 基因组学和生物信息学
  • 计算生物学 计算生物学

背景情况:

  • 单细胞RNA测序 (scRNA-seq) 揭示了细胞异质性,但遭受了技术上的"脱落零".
  • 现有的归算方法通常会导致低或过度归算,扭曲scRNA-seq数据的解释.

研究的目的:

  • 为scRNA-seq数据开发一个精确平衡的归算 (PbImpute) 方法.
  • 为了在恢复丢失的数据和保持真正的生物零点之间实现最佳平衡.

主要方法:

  • PbImpute集成了零膨胀负二项式 (ZINB) 建模与静态和动态修复算法.
  • 它采用多阶段的归算,包括参数优化,静态修复,二次丢失识别,图形嵌入神经网络和动态修复.

主要成果:

  • PbImpute 在区分从生物零点 (F1 评分 = 0.88) 开始的技术学中表现优异.
  • 该方法显著改善了基因-基因和细胞-细胞相关性,差异表达分析和集群可视化.
  • 废弃性研究证实了归算和修复模块的有效性.

结论:

  • PbImpute为scRNA-seq数据归算提供了一个平衡的方法,减少信号扭曲和保存生物信号.
  • 这种方法提高了细胞亚群识别和差异基因表达分析的准确性,推进了细胞异质性研究和疾病研究.