通过乳酸积累产生广泛的抗菌活性,具有BTS1-KNOCK-OUT的Saccharomyces cerevisiae
Liu Cong1, Yuan Zhou1, Yu Zhang1
1School of Medical Technology, Xuzhou Medical University, Xuzhou, Jiangsu, China.
Frontiers in cellular and infection microbiology
|February 17, 2025
概括
BTS1-knockout 麦芽菌 (Saccharomyces cerevisiae) 对大肠杆菌和金黄色杆菌等病原体表现出广泛的抗菌活性. 这种有前途的益生菌抑制细菌生长和生物膜形成,具有低毒性,为抗生素提供了潜在的替代品.
科学领域:
- 微生物学 微生物学
- 益生菌 益生菌是一种益生菌.
- 药物耐药性 药物耐药性 药物耐药性
背景情况:
- 抗生素耐药性是一个日益严重的全球健康危机.
- 谨慎使用抗生素对于控制细菌感染至关重要.
- 益生菌提供了一种潜在的策略来对抗致病细菌.
研究的目的:
- 为了研究BTS1-knockout (BTS1-KO) 的抗菌活性Saccharomyces cerevisiae.
- 为了评估其在体外和体外对病原性细菌的有效性.
- 探索其抗菌作用的潜在机制.
主要方法:
- 最低抑制度 (MIC) 测定,生长曲线和细菌静止谱分析.
- 共同培养实验和生物膜形成测试对抗大肠杆菌和金黄色杆菌.
- 使用Galleria mellonella感染模型和机制性调查的体内研究.
主要成果:
- BTS1-KO S. cerevisiae有效地抑制了病原体的生长和生物膜的形成.
- 它表现出有利的益生菌特性,包括抗酸和胆盐.
- 在体内研究显示,感染Galleria mellonella幼虫的生存时间显著延长.
- 由BTS1-KO S. cerevisiae产生的乳酸被确定为一个关键的抑制机制.
结论:
- BTS1-KO S. cerevisiae在体外和体内表现出显著的广泛抗菌活性.
- 它的低毒性和有利的益生菌特性使其成为预防和控制细菌感染的有希望的候选人.
- 这项研究强调了工程益生菌作为传统抗生素替代品的潜力.
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