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TREM-1作为诺罗病毒发病的潜在核心受体:从转录基因分析和分子对接的见解
Mike Telemaco Contreras Colmenares1, Amanda de Oliveira Matos1, Pedro Henrique Dos Santos Dantas1
1Laboratório de Imunologia de Mucosas e Imunoinformática, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia 74605-170, Brazil.
ACS omega
|February 17, 2025
概括
在骨髓细胞1 (TREM-1) 中表达的收费类受体可能在诺罗病毒 (NoV) 感染中发挥作用. 这项研究发现TREM-1与NoV VP1蛋白相互作用,表明它与疾病有关.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 生物信息学是一种生物信息学.
背景情况:
- 诺罗病毒 (NoV) 引起急性腹疾病,但由于培养困难,其宿主相互作用的研究具有挑战性.
- 在骨髓细胞1 (TREM-1) 中表达的托尔类受体与病毒感染期间放大炎症反应有关.
研究的目的:
- 调查TREM-1在诺罗病毒感染和病变发生中的潜在参与.
- 为了阐明TREM-1和诺罗病毒蛋白之间的分子相互作用.
主要方法:
- 对公共转录组数据的分析,以评估在小鼠诺病毒 (MNoV) 感染期间的TREM-1表达.
- 分子对接和动力学模拟用于模拟小鼠和人类TREM-1和MNoV/NoV VP1蛋白之间的相互作用.
- 在MNoV感染期间对TREM-1与亡和烧亡途径基因的同时表达分析.
主要成果:
- 在MNoV感染期间观察到Trem1和Trem3的表达增加.
- 穆林TREM-1识别了MNoV VP1.1的C'-D'部分.
- 人类TREM-1在不同年份显示与NoV GII.4 VP1蛋白段 (S和P) 保持相互作用,由CDR3区域介导.
- 在MNoV感染期间,Trem1主要与热道基因共同表达.
结论:
- TREM-1 与诺罗病毒VP1蛋白相互作用,这表明它参与了NoV病变的发生.
- 在诺罗病毒感染中,TREM-1 可能起到核心受体的作用.
- 在不同病毒菌株和时间段中,TREM-1和NoV之间的相互作用是保留的.
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