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Updated: May 27, 2025

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

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基于PCR的定量试验,用于测量子细胞生成细胞模型中的声波子信号

Priyadarshini Halder1, Shubhra Majumder2

  • 1CellBio Lab, Institute of Health Sciences, Presidency University.

Journal of visualized experiments : JoVE
|February 17, 2025
PubMed
概括

这项研究提出了一种新的定量PCR方法,通过测量静止细胞中的Sonic Hedgehog通路基因表达来评估初级乳毛功能. 这种测定有助于理解由初级毛缺陷引起的毛病.

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相关概念视频

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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