用有机电化学晶体管翻译细胞外电子转移活动
Yang Gao1, Yuchen Zhou2, Xudong Ji3
1McKetta Department of Chemical Engineering, University of Texas at Austin.
Journal of visualized experiments : JoVE
|February 17, 2025
概括
使用有机电化学晶体管 (OECT) 将微生物细胞外电子转移 (EET) 转化为电信号. 这种新型的接口使微生物生物传感和生物计算应用成为可能.
科学领域:
- 微生物学 微生物学
- 生物电子学 生物电子学
- 合成生物学 合成生物学
背景情况:
- 细胞外电子转移 (EET) 将微生物新陈代谢与环境联系起来,其中包括Geobacter和Shewanella的关键研究模型.
- 在发酵和人类肠道微生物群中,越来越多地发现了EET活性物种.
- 跨越生物和电子系统为新型应用提供了潜力.
研究的目的:
- 使用有机电化学晶体管 (OECT) 将微生物ETE活动转化为可检测的电信号的协议开发.
- 为了证明混合OECT-微生物系统的生物传感和生物计算能力.
- 在生物系统和电子之间建立一个新的接口.
主要方法:
- 使用的有机电化学晶体管 (OECT) 具有多3,4-乙烯二氧化:多硫酸 (PEDOT:PSS) 通道.
- 通过 Shewanella oneidensis.证明了OECT通道的ET驱动的除兴奋剂.
- 通过生物传感的遗传电路使用ETE流的转录控制.
- 在微生物细胞内集成的基于等离子体的布尔逻辑门用于信号处理.
主要成果:
- 通过OECT成功地将微生物ET活动转化为可测量的电信号.
- 通过通过基因电路控制ET流来展示化学刺激的生物感知.
- 通过使用逻辑门对环境信号进行细胞处理,证明了生物计算潜力.
- 建立了微生物ETT和电子设备之间的功能接口.
结论:
- 开发的基于OECT的系统有效地将微生物ETE转化为电信号.
- 这种混合系统具有高通量选,生物传感和生物计算的巨大潜力.
- 这项工作为将生物系统与电子元件连接开辟了新的途径.
相关概念视频
Interfacial Electrochemical Methods: Overview
216
Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current...
216
Electromotive Force
25.7K
Electricity is generated by either electrons or ions flowing through a solution or a conducting medium. This flow of electrons or specifically electrical charge is defined as an electric current. When electrons move through a wire, they generate an electric current. It can be recalled that in a redox reaction, electrons are lost and gained. In the spontaneous redox reaction of zinc with copper, when zinc is immersed in a copper ion solution, a transfer of electrons from one...
25.7K
Electrolysis
25.9K
In a galvanic cell, the electrical work is done by a redox system on its surroundings as electrons produced by the spontaneous redox reactions are transferred through an external circuit. Alternatively, an external circuit does work on a redox system by imposing a voltage sufficient to drive an otherwise nonspontaneous reaction in a process known as electrolysis. For instance, recharging a battery involves the use of an external power source to drive the spontaneous (discharge) cell reaction in...
25.9K
The Electron Transport Chain
16.0K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
16.0K
Electron Transport Chains
97.0K
The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
The ETC is comprised of...
97.0K
Potentiometry: Membrane Electrodes
406
Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
406


