二维猪肠道器官体,反映了原生肠道的生理性质
Pascal Benz1, Masina Plenge2, Simon Wagner2
1Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover; pascal.benz@tiho-hannover.de.
Journal of visualized experiments : JoVE
|February 17, 2025
概括
研究人员开发了2D猪肠道有机体培养物来研究胃肠道 (GIT) 功能. 这种符合3R标准的模型为生理学研究提供了对上皮表面的增强访问.
科学领域:
- 胃肠道学和生理学
- 在 Vitro 模型中
- 动物替代品 动物替代品
背景情况:
- 胃肠道 (GIT) 对于消化,营养吸收和病原体防御至关重要.
- 传统的GIT研究动物模型面临着伦理和准确性的局限性,推动了对符合3R的替代品的需求.
- 与传统细胞培养相比,猪器官提供了更具生理相关性的体外模型.
研究的目的:
- 开发和表征二维 (2D) 培养猪内和结肠器官在穿孔插件上的二维 (2D) 培养.
- 建立一个强大的体外模型来研究猪胃肠道生理学,遵循3R原则.
- 为了在猪肠道模型中详细地表皮质运输过程的生理特征.
主要方法:
- 猪内和结肠的二维器官培养的发展有两个阶段:单层形成和细胞分化.
- 通过测量体电阻 (TEER) 来监测细胞生长和屏障完整性.
- 使用乌辛室技术实时量化和表征电源传输过程,如化物分泌.
主要成果:
- 成功建立了2D猪直肠和结肠器官培养物.
- 在结肠的第8天和脏器官的第16天实现了TEER稳定,表明成熟的上皮屏障.
- 在开发的有机体模型中,证明了使用Ussing腔室来分析活性电基运输的可行性.
结论:
- 开发的2D猪器官模型为GIT研究提供了一个生理相关且符合3R的体外平台.
- 这种模型有助于详细描述上皮质运输机制,克服了传统有机体培养的局限性.
- 该模型有可能用于研究胃肠道病理生理学和开发治疗策略.
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