开发和验证基于转录因子监管网络的签名,用于肺腺癌的个性化预后风险
Kai Wang1,2, Jun Xiang2, Jun Zhou2
1Department of Epidemiology, School of Public Health, Southeast University, Nanjing, Jiangsu, China.
International journal of cancer
|February 17, 2025
概括
一个新的预后签名,单细胞基因对 (scGPS),准确地预测肺腺癌 (LUAD) 患者的整体存活率. 这种签名改善了风险分层,并有助于为LUAD提供个性化治疗策略.
科学领域:
- 在瘤学瘤学.
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 肺腺癌 (LUAD) 存在高复发风险和低生存率,尽管有进展.
- 准确的预后签名对于LUAD患者有效的风险分层至关重要.
研究的目的:
- 开发和验证肺腺癌 (LUAD) 的新型预后特征,以预测整体存活率 (OS).
- 确定关键的转录因子和参与LUAD进展的基因调控网络.
主要方法:
- 利用单细胞RNA测序 (scRNA-seq) 来识别与表皮细胞到介质细胞转换 (EMT) 相关的表皮细胞特异性转录因子.
- 构建了一个单细胞基因对 (scGPS) 签名,包括来自调控网络的3521个基因对.
- 通过大量RNA-seq和微阵列数据验证了23个LUAD队列 (2740名患者) 的scGPS签名.
主要成果:
- 该scGPS签名有效地将LUAD患者分为高风险和低风险组,具有显著不同的OS (HR=1.78).
- 与现有签名相比,scGPS表现出优越的性能,并保持了独立数据集的预测能力,包括早期的LUAD.
- 这种特征被证明是一个独立的预后因素,并且在与瘤阶段相结合时,提高了准确性.
结论:
- scGPS签名为LUAD患者的个性化预后提供了一个强大的工具.
- 这种特征具有显著的临床应用潜力,用于 LUAD 的风险分层和治疗规划.
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