在中国的野猪中识别和基因分析新的短暂病毒
Zhongzhong Tu1, Tong Wang2, Yu Xu3
1Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratory of Pathogen and Biosecurity, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, PR China.
Virologica Sinica
|February 17, 2025
概括
在中国各地的野猪中发现了新的短暂病毒 (EV). 从受感染的猪身上收集的也呈阳性,这表明它们在EV传播中的作用.
科学领域:
- 病毒学 病毒学
- 野生动物健康 野生动物健康
- 关节动物传播疾病 关节动物传播疾病
背景情况:
- 埃菲马病毒 (EV) 是关节动物传播的拉布病毒,主要存在于牛和关节动物中.
- 最近的发现在家养猪中发现了新的EV,扩大了它们已知的宿主范围.
- 野猪种群中EVs的存在和多样性在很大程度上仍然没有特征.
研究的目的:
- 调查中国野猪种群中短暂病毒的存在和遗传多样性.
- 为了确定涉及在野猪种群内传播EVs的潜在载体.
- 描述新型埃菲梅罗病毒菌株及其遗传学关系.
主要方法:
- 在野猪样本中的EVs的初始检测中采用了元转录组 (MTT) 测序.
- 特殊的逆转录嵌套聚合酶链反应 (RT-nPCR) 用于进一步查组织样本.
- 进行了全基因组测序,以获得已识别的野猪短暂病毒 (WbEVs) 的完整基因组序列.
主要成果:
- 在中国四个省份的五只野猪中检测到ephemerovirus.
- 从两个受感染的野猪收集的也对EVs呈阳性.
- 获得了四个完整的WbEV基因组序列,揭示了两种新型EV物种和两种与猪病毒2 (PoEV2) 相关的菌株.
结论:
- 野猪有各种各样的短暂病毒,扩大了这些病毒已知的宿主范围.
- 可能在野猪种群中EVs的自然循环和传播中发挥作用.
- 需要进一步研究野生猪中EV感染的生物学影响.
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