二碳酸诱导了微藻外细胞聚合物质的增强生产及其表征
Serveshwar Dutt Tripathi1, Romit Mitra2, Samridhi Kulshrestha1
1Department of Bioscience & Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur - 721302, Paschim Medinipur, West Bengal, India.
Bioresource technology
|February 17, 2025
概括
这项研究优化了Chlorella vulgaris的培养,以提高细胞外聚合物质 (EPS) 的产生. 较高的二碳酸盐水平提高了EPS产量和微藻自流体,提供了可扩展的碳捕获方法.
科学领域:
- 生物技术是生物技术.
- 环境科学 环境科学
- 微生物学 微生物学
背景情况:
- 菌产生细胞外聚合物物质 (EPSs),有利于自我定位和抗压力.
- 优化生物过程用于EPS生产对于微藻应用至关重要.
- 溶解的无机碳来源,如二碳酸盐,影响微藻生长和EPS合成.
研究的目的:
- 调查不同度的二碳酸对Chlorella vulgaris生长和EPS生产的影响.
- 确定最大限度地提高EPS产量和微藻自流体的最佳条件.
- 评估这一战略对二氧化碳捕获的潜力.
主要方法:
- 在经过修改的Bold's基底介质中种植Chlorella vulgaris,其初始度不同于二碳酸.
- 使用光生物反应器控制藻类生长.
- 量化生物质度,生产力和EPS收益率.
- 描述EPS的特性,包括自动花和分子量.
主要成果:
- 最佳生物质度 (1.60 g/L) 和生产率 (0.19 g/L/d) 在130 mM二碳酸盐下实现.
- 在 190 mM 碳酸盐下获得了显著更高的 EPS 产量 (183 mg/g 生物质),增加了 5 倍.
- 在 190 mM 碳酸盐下,EPS 在 8 小时内促进了微藻最大自流体.
- 鉴定到的EPS是一种具有1400kDa分子量的糖蛋白.
结论:
- 碳酸的度是优化Chlorella vulgaris EPS生产和微藻自我定位的关键因素.
- 开发的生物工艺提高了EPS产量,并提供了一种可扩展的二氧化碳从烟气中捕获的潜在方法.
- 这种方法将微藻生物技术与环境修复策略相结合.
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