通过固体和液体发酵优化内胞生产,以开发基于Bacillus velezensis的产品的有效配方
Laís Mayara Melo Duré1, Gabriel Moura Mascarin2, Wagner Bettiol3,4
1Departamento de Proteção de Plantas, Faculdade de Ciências Agronômicas, UNESP, Botucatu, SP, CEP 18610-034, Brazil.
概括
使用发酵优化了Bacillus velezensis AP-3内,在粉末配方中显示出高稳定性. 这种生物控制剂有效地促进了植物生长,并抑制了可持续农业的真菌病原体.
科学领域:
- 农业微生物学 农业微生物学
- 植物病理学 植物病理学
- 生物技术是生物技术.
背景情况:
- 百菌 velezensis是一种有益的细菌,在农业中具有潜在的应用.
- 内体为微生物注射剂提供了增强的稳定性.
- 优化发酵和配方对于有效的生物控制和植物生长促进至关重要.
研究的目的:
- 使用液体和固态发酵优化Bacillus velezensis AP-3内体的生产.
- 评估内胞粉末配方的稳定性.
- 评估配方内的植物生长促进和生物控制能力.
主要方法:
- 使用米粉和土豆粉进行固体发酵;使用糖蜜和棉花粉进行浸泡发酵.
- 用塔尔克和土豆粉配制内体.
- 在豆植物上进行植物生长促进试验和体外抑制试验对抗Sclerotinia sclerotiorum和Fusarium oxysporum.
主要成果:
- 固态发酵产生了高达2.06 × 10^8 CFU/g的产量,而浸泡发酵产生了1.7 × 10^9 CFU/mL的产量.
- 粉末配方在室温下维持了226天的活力.
- B. velezensis AP-3 显著抑制了真菌病原体,并增强了豆植物根的发育.
结论:
- 优化的发酵策略显著增加了Bacillus velezensis AP-3内胞产量.
- 稳定的粉末配方可延长内的保质期,用于实际的农业应用.
- 细菌 velezensis AP-3 作为生物控制和植物生长促进的双重作用剂,支持可持续的农业实践.
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