在玉米泛基因组中,附件基因家族的进化,变异和表达模式
Xin Liu1,2, Minghu Zhang2, Xin Zhao1,2
1Sichuan Oil Cinnamon Engineering Technology Research Center, Yibin, 644000, Sichuan, China.
Scientific reports
|February 17, 2025
概括
玉米附属素 (Ann) 基因对植物发育和应激反应至关重要. 全基因组分析揭示了12个ZmAnn基因,其中一些基因受到积极选择,突出了遗传多样性,为未来的研究提供了基础.
科学领域:
- 植物遗传学和基因组学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 附件蛋白 (Anns) 是依赖的脂结合蛋白,对植物生长,发育和应激反应至关重要.
- 以前对玉米附录素 (ZmAnn) 基因的研究受限于单基因组参考.
研究的目的:
- 使用高质量的玉米泛基因组对ZmAnn基因进行全面的生物信息分析.
- 研究ZmAnn基因的进化动态,结构变异和表达模式.
主要方法:
- 对26个高质量的玉米基因组进行泛基因组分析,以识别Ann基因.
- 评估Ka/Ks比率以评估选择压力.
- 遗传学分析来对ZmAnn蛋白进行分类.
- 在冷应力下进行转录组分析和加权基因共同表达网络分析 (WGCNA).
主要成果:
- 确定了12个ZmAnn基因 (9核,3近核),揭示了单基因组研究的局限性.
- ZmAnn10在一些品种中显示出正选择;其他品种经历了净化选择.
- 遗传学分析将ZmAnn蛋白质分为六个类.
- 结构变异创造了非典型的ZmAnn基因.
- 对于不同的ZmAnn成员来说,观察到不同的组织特异性和应激反应表达模式.
- 四个ZmAnn基因 (ZmAnn2,ZmAnn6,ZmAnn7,ZmAnn9) 与冷应激联合表达模块有关.
结论:
- 玉米泛基因组为ZmAnn基因家族的多样性和演变提供了更完整的画面.
- ZmAnn 基因表现出多样化的进化轨迹和表达特征,表明它们具有专门的作用.
- 这项研究为ZmAnn基因在玉米改善和应激弹性方面的功能性表征奠定了基础.
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