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Updated: May 27, 2025

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
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Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

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超声波辅助的蛋白质提取改善了从瘤组织中膜结合和DNA结合蛋白质的蛋白质检测

Qing Kay Li1, T Mamie Lih2, David J Clark2

  • 1Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA. qli23@jhmi.edu.

Nature protocols
|February 17, 2025
PubMed
概括

这项研究通过将超声波添加到组织溶解中来增强蛋白质组分析,改善了关键蛋白质的检测. 更新后的协议为基于质谱的研究提供了最佳条件.

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相关概念视频

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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