从Achyranthes aspera L.中分离的α-spinasterol通过NF-κB和Nrf2/HO-1通路改善了炎症
Qiongli Zeng1,2, Weiting Xiao1,2, Heng Zhang1,2
1School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China.
Scientific reports
|February 17, 2025
概括
这项研究确定了来自Achyranthes aspera的α-spinasterol作为一种强大的抗炎剂. 它通过调节关键信号通路,有效地减少细胞中的炎症标记物.
科学领域:
- 植物化学 植物化学
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 以前的研究表明,Achyranthes aspera L. (A. aspera) 的低极部分具有显著的抗炎活性.
- 识别和分离关键的生物活性化合物对于理解这种活动至关重要.
研究的目的:
- 为了分离和识别具有抗炎性质的A. aspera的化合物.
- 为了研究α-spinasterol在脂聚糖 (LPS) 诱导的RAW264.7细胞中的抗炎机制.
主要方法:
- 使用色谱和光谱技术分离和结构识别化合物.
- 高性能液体染色学 (HPLC) 用于在不同季节的A. aspera中量化α-spinasterol含量.
- 在体外评估LPS诱导的RAW264.7细胞中的抗炎作用,测量细胞因子水平和蛋白质表达.
主要成果:
- 三种化合物被分离和鉴定出来:α-spinasterol (1),7,8-dihydrospinasterol (2) 和22,23-dihydrospinasterol (3).
- 根据HPLC测定,A. aspera的α-spinasterol含量在春季和冬季是最高的 (0.00850.0157%).
- α-spinasterol显著降低了IL-6,PGE2和TNF-α水平,抑制了COX-2,5-LOX,p-IKKβ,p-NFκB和p-IkBα的表达,并在LPS治疗的细胞中促进了Nrf2,HO-1和NQO1的表达.
结论:
- α-spinasterol在抑制RAW264.7细胞中LPS诱导的炎症方面显示出显著的潜力.
- 抗炎机制包括抑制NF-κB通路和激活Nrf2通路.
- α-spinasterol有效地减少炎症因子的过度释放,突出其治疗前景.
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