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功能性阿普塔默在体外蛋白质-蛋白质相互作用阻断的进化

Tongxuan Wei¹, Qinguo Liu¹, Jun Li¹

¹State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.

Analytical chemistry

|February 18, 2025

概括

功能性阿帕特默选择得到了FAIVE的增强,FAIVE是一种将序列功能与光联系起来的新方法. 这种方法有效地识别出aptamers,例如那些抑制SARS-CoV-2尖端蛋白和 hACE2相互作用的aptamers.

科学领域:

生物技术和分子生物学
生物化学和生物物理学

背景情况:

适应器应用已经扩展到功能能力的高亲和度之外.
传统的SELEX方法用于功能性阿帕特默识别是耗时和劳动密集的.
较高的阿普坦酶亲和度并不能保证最佳的功能.

研究的目的:

引入一种新的查方法,即功能性体体在体外演化 (FAIVE),以有效地识别功能性体.
为了证明FAIVE在获得抑制蛋白质-蛋白质相互作用的aptamers方面的有效性.
提供理论见解,用于验证对aptamer选择的珠子库质量.

主要方法:

在体外演化 (FAIVE) 中开发和实施功能性阿巴,这是一种将阿巴序列功能与光强度联系起来的方法.
应用FAIVE以获得针对SARS-CoV-2尖端受体结合域 (RBD) 和 hACE2相互作用的修饰DNA吸收体.
模拟了乳液PCR过程,以调查验证珠子库质量的标准.

主要成果:

FAIVE成功识别了能够破坏SARS-CoV-2 RBD-hACE2相互作用的修饰DNA吸收体,证明了蛋白质-蛋白质相互作用的抑制.
该研究提供了通过乳液PCR建模对珠子库质量验证的理论见解.
FAIVE 便于识别具有多种功能的体.

结论:

FAIVE在aptamer选方面取得了重大进展,直接将序列功能与可测量的信号联系起来.
这种方法加速了功能性体的发现,包括那些与SARS-CoV-2等病毒标相关的蛋白质-蛋白质相互作用抑制的功能性体.
FAIVE 概念可以适应各种研究环境,用于识别具有不同功能能力的体.

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