分析性能的评估和优化血清25(OH) DLC-MS/MS测量
Weiyan Zhou1, Meiliang Gong2, Yuanli Mao3
1National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/ National Center of Gerontology, Beijing, China.
Clinical chemistry and laboratory medicine
|February 18, 2025
概括
一种优化的液体染色学-并联质谱法 (LC-MS/MS) 方法显著提高了中国临床实验室血清25-基维生素D (25(OH) D测量的准确性和可靠性. 这种增强的方法减少了实验室间的差异,从而导致更可靠的诊断结果.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 血清25-基维生素D (25(OH) D) 的测量对于临床诊断至关重要.
- 当前方法的实验室间变化可能导致诊断错误.
- 对于可靠的患者护理,25...OH) D测定标准化至关重要.
研究的目的:
- 在中国临床实验室中评估内部液体染色学-并联质谱法 (LC-MS/MS) 测量血清25(OH) D的方法的性能.
- 提出和验证一个优化的LC-MS/MS方法,以提高准确性和可靠性.
主要方法:
- 十二家中国顶级临床实验室使用现有的内部LC-MS/MS方法和新优化的LC-MS/MS方法分析了血清池.
- 使用方差系数 (CV) 评估精度和使用偏差对比NIST参考测量程序 (RMP) 的真实性.
- 对比了两种方法的分析性能,包括测量不确定性 (MU).
主要成果:
- 与内部方法相比,优化方法显著提高了实验室满足25[OH]D2和25[OH]D3性能标准的百分比 (例如,80%对25[OH]D2CV的43%).
- 精确度 (CV) 和真实性 (偏差) 都通过优化方法得到了增强.
- 测量不确定性 (MU) 显著降低,尽管低度样本仍然存在挑战.
结论:
- 优化的LC-MS/MS方法明显提高了血清25(OH) D的精度,准确性和测量不确定性.
- 需要进一步努力,以确保在中国的所有临床实验室中保持一致的可靠性和准确性.
- 对于准确的临床决策,25...OH) D测量的标准化至关重要.
相关概念视频
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