保存的开花基因的对抗cis-调控元素介导着发育强度
Amy Lanctot1,2, Anat Hendelman1,2, Peter Udilovich2
1HHMI, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724.
概括
植物花的发育依赖于精确的基因控制. 研究人员设计了番茄异常植物器官 (UFO) 基因调节变异,揭示了对物种之间发育强度至关重要的保存的 cis 调节元素.
科学领域:
- 植物发育生物学 植物发育生物学
- 分子遗传学 分子遗传学
- 进化发育生物学 进化发育生物学
背景情况:
- 精确的基因表达的时间和空间控制对于植物的发育过渡至关重要,特别是对于花的形成.
- F-box基因 - - 非寻常的花器官 (UFO) - - 在植物种类中协调这些转变.
- 研究UFO的Cis调节为我们提供了对塑造关键发育基因的进化过程的洞察.
研究的目的:
- 探索控制番茄中非常见的花器官 (UFO) 基因的 cis 调节机制.
- 设计cis-regulatory等位基因并分析它们对花发育的影响.
- 为了比较UFO在番茄和Arabidopsis中的监管保护和功能分歧.
主要方法:
- 在一个可访问的染色体区域内,在番茄UFO正义词中识别一个保存的促进子序列.
- 在体内对cis调节性等位基因进行工程,以诱导功能丧失和功能增加的花表型.
- 对转录因子结合点和等位基相互作用的分析;与Arabidopsis UFO调节的比较研究.
主要成果:
- 番茄UFO中的工程 cis-调节性等位基因导致了明显的花缺陷,与中断的转录因子结合部位有关.
- 对立基因之间的剂量依赖相互作用影响了表型表达性和透性,突出了UFO表达剂量的重要性.
- 阿拉比多普西斯UFO促进者的同类调节序列虽然分散,但对花发育保持类似的控制,但由于表达模式的变化,具有不同的表型结果.
结论:
- 强大的番茄花的发展需要精确的时间控制UFO表达剂量.
- 短暂的,保存的 cis-regulatory 序列,招募激活和抑制机器,对于保持发育强度至关重要.
- 在UFO表达模式的进化分歧有助于物种之间不同的监管效应,尽管保留了监管元素.
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