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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: May 4, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

描述最近PDMS在基于电动力学的微流体设备的性能和制造中的变化,用于细胞分类应用.

Alexandra R Hyler¹, Dean E Thomas¹, Kyle S Kinskie¹

¹CytoRecovery, Inc., Blacksburg, Virginia, USA.

Electrophoresis

|February 18, 2025

概括

研究人员在微流体设备中发现了意想不到的PDMS物质变化,导致泡形成和细胞死亡. 强大的质量控制和泡清除协议使得细胞分类性能保持一致,这对于商业化至关重要.

关键词:

细胞分类细胞分类在商业化,商业化.电动运动学电动运动学微型制造是指微型制造.微流体学在微流体学方面

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Published on: February 23, 2017

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Last Updated: May 4, 2026

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科学领域:

生物技术是生物技术.
材料科学材料科学材料科学
细胞生物学细胞生物学

背景情况:

微流体设备提供精确的细胞分类,没有标签.
微流体设备的商业化受到制造挑战的限制.
无接触介电泳 (cDEP) 是一个有前途的无标签细胞丰富技术.

研究的目的:

研究一种无标签的cDEP微流体装置,用于细胞亚群丰富.
确定影响设备性能的动态泡形成的根本原因.
评估扩展cDEP设备制造的可行性.

主要方法:

制造,环境和实验变量的系统评估.
聚二甲基西洛 (PDMS) 材料的化学分析.
用于设备制造的替代聚合物的比较.
开发用于cDEP设备的泡清除协议.

主要成果:

意想不到的泡形成导致近100%的细胞死亡在几分钟内.
调查的变量,包括替代聚合物,没有解决这个问题.
化学分析显示PDMS的特性和组成发生了变化,这是原因.
开发的协议实现了高于90%的细胞存活率的一致性能.

结论:

改变PDMS属性是影响cDEP设备性能的一个关键因素.
强大的质量控制和泡管理对于可靠的微流体细胞分类至关重要.
解决PDMS材料的一致性是商业化cDEP技术的关键.