开发一种双感应色度探针,用于测量总抗氧化剂容量,使用铁(III) -o-phenanthroline试剂进行测量
Ervanur Elmas1, Furkan Burak Şen2, Mustafa Bener2
1Department of Chemistry, Faculty of Science, Istanbul University, Fatih, 34134, Istanbul, Türkiye; Institute of Graduate Studies in Science, Istanbul University, Fatih, 34134, Istanbul, Türkiye.
Talanta
|February 18, 2025
概括
开发了一种使用Fe(III) -phenanthroline (Fe(III) -phen的新型双模式传感系统,用于总抗氧化能力 (TAC) 分析. 这种创新方法提供了一种简单,快速和灵敏的方法来确定各种样本中的抗氧化剂水平.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 准确确定总抗氧化能力 (TAC) 对于评估氧化应激和抗氧化化合物的有效性至关重要.
- 现有的TAC测定方法往往涉及多个步骤或试剂,限制了其速度和简单性.
- Fe(III) - 芬罗林 (Fe(III) - ) 试剂具有独特的氧化还原特性,可用于传感应用.
研究的目的:
- 开发一种双模式传感系统,用于定量确定总抗氧化能力 (TAC).
- 通过两个不同的检测机制,利用Fe(III) - 芬罗林 (Fe(III) - ) 试剂.
- 建立一种简单,快速和敏感的TAC分析方法,适用于各种矩阵.
主要方法:
- 一个双模式传感系统的设计是基于Fe(III) -phenanthroline (Fe(III) -phen) 试剂.
- 机制1:通过510nm的吸收量量化了Fe(III) -phen被抗氧化剂减少到Fe(II) -phen的数量.
- 机制2:利用Fe(III) -phen的氧化酶类活性产生超氧化基,通过652 nm的TMB氧化监测抗氧化抑制,产生超氧化基.
主要成果:
- 该系统成功地确定了标准抗氧化剂,合成混合物和食品提取物中的TAC.
- 获得了trolox (TR) 的线性校准图,其方程为:A510 = 0.0221CTR + 0.0223和 ΔA = 0.0301CTR + 0.0583.
- 托洛克斯的检测极限 (LOD) 为0.45μM (基于减少) 和0.87μM (基于TMB).
结论:
- 开发的双模式传感系统有效地使用单个Fe(III) -phen探针来确定总抗氧化能力 (TAC).
- 该系统在不同的样本类型中显示出高灵敏度和适用性,包括复杂的食品矩阵.
- 这种创新方法为TAC分析提供了一种简单,快速和敏感的方法,推动了抗氧化剂研究.
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