循环RNA的降解由核糖核酶DIS3进行
Xiao Tao1, Si-Nan Zhai2, Chu-Xiao Liu1
1Key Laboratory of RNA Innovation, Science and Engineering, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
Molecular cell
|February 18, 2025
概括
酶DIS3降解循环RNAs (circRNAs),这一过程对于调节它们的水平至关重要. 这一发现揭示了独立于RNA外体的circRNA周转的关键途径.
科学领域:
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
- 生物化学 生物化学
背景情况:
- 循环RNAs (circRNAs) 是通过反向拼接形成的,并且可以抵抗线性RNA衰变.
- 在正常条件下, circRNA 降解的一般途径尚未得到很好的定义.
研究的目的:
- 为了确定负责circRNAs降解的酶.
- 阐明circRNA循环的机制和调节.
主要方法:
- 在真核细胞中,内核分解酶DIS3的耗尽.
- 使用测序分析circRNA和线性RNA水平的分析.
- 研究DIS3的酶活性和RNA外体组合物的作用.
- 通过DIS3.3降解的环RNA的序列丰富分析.
- 评估合成RNA圈的稳定性,具有不同的动机.
主要成果:
- DIS3的耗尽导致超过60%的环RNAs的上调,对线性RNAs的影响最小.
- DIS3介导的circRNA降解在物种中保持,发生在细胞质中,并需要DIS3的内核分解活性.
- 这种降解途径与RNA外体复合体独立.
- DIS3优先降解含有U丰富基因的circRNAs,通过合成RNA圆的稳定性测试得到证实.
结论:
- 内核分解酶DIS3是circRNAs降解的一个关键因素.
- DIS3调解了用于circRNA周转的保存细胞质路径,更喜欢富含U的序列.
- 这些发现建立了调节circRNA稳定性和丰度的一般机制.
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