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在口腔粘膜细胞中TMPRSS2的表达是由被转染的双链RNA和IL-1β诱导的

Misaki Akagi¹, Kouji Ohta², Miyuki Sakuma¹

¹Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.

Journal of oral biosciences

|February 18, 2025

概括

口腔粘膜细胞表达转膜血清蛋白酶2 (TMPRSS2),这是病毒进入的关键因素. 干白素-1β (IL-1β) 和病毒核酸显著增加TMPRSS2的表达,促进感染.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

跨膜血清蛋白酶2 (TMPRSS2) 对于病毒进入宿主细胞至关重要,包括SARS-CoV-2和流感A.
提升的TMPRSS2表达是病毒感染传播的危险因素.
了解口腔粘膜细胞中的TMPRSS2调节对于控制病毒感染至关重要.

研究的目的:

研究TMPRSS2在口腔角质细胞和纤维细胞中的表达.
确定诱导这些口腔粘膜细胞TMPRSS2表达的因素.
阐明IL-1β和病毒核酸在TMPRSS2诱导中的作用.

主要方法:

在口腔角质细胞 (RT7) 和纤维细胞 (GT1) 中检查了构成TMPRSS2的mRNA和蛋白质表达.
分析了与核酸 (dsRNA,dsDNA) 和炎症性细胞因子 (IL-1β) 发生传染后的TMPRSS2诱导.
研究了IL-1β对STAT1激活与dSRNA诱导的TMPRSS2表达有关的影响.

主要成果:

口腔角质细胞和纤维细胞构成性地表达TMPRSS2.
双链RNA (Poly(I:C)) 和双链DNA (poly(dA:dT)) 增加了TMPRSS2的表达.
IL-1β显著提高了TMPRSS2的调节,特别是与Poly (I:C) 结合,并增强了Poly (I:C) 激活的STAT1信号传递.

结论:

表达TMPRSS2的口腔角质细胞和纤维细胞对SARS-CoV-2和A型流感敏感.
IL-1β与病毒成分合作,增强TMPRSS2的表达,促进病毒侵入口腔粘膜.
在口腔粘膜细胞中准TMPRSS2可能是预防病毒感染的策略.

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