相关实验视频

Updated: May 27, 2025

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

Published on: September 20, 2016

BMAL1 调节子宫和乳腺肌瘤中的原蛋白生产

Yidan Dai¹, Masanori Ono², Tomoo Suzuki¹

¹Department of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, 160-0023, Japan.

Reproductive sciences (Thousand Oaks, Calif.)

|February 18, 2025

概括

这项研究揭示了昼夜钟基因BMAL1 (大脑和肌肉的aryl-hydrocarbon-receptor-nuclear-translocator-like protein-1) 影响人类子宫组织和乳腺瘤中的原蛋白产生,影响细胞迁移和入侵.

科学领域:

生殖生物学生殖生物学
时间生物学时间生物学
分子妇科学分子妇科学

背景情况:

时钟异常与动物不育有关,但对人类的数据很少.
循环时钟基因调节生理过程,包括生殖功能.

研究的目的:

研究生物钟基因的表达,特别是BMAL1 (大脑和肌肉的aryl-hydrocarbon-receptor-nuclear-translocator-like protein-1) 的表达,在人类肌肉膜和乳腺瘤 (LM) 中.
分析BMAL1对人类子宫和LM中的原合成基因的影响.
探索BMAL1在肌肉细胞和乳腺瘤细胞细胞行为中的作用.

主要方法:

对人类肌肉膜和肌肉膜组织的分析.
免疫组织化学,细胞培养,免疫光和siRNA转染.
定量实时PCR,伤和穿孔测试.

主要成果:

在人类肌肉膜和LM细胞中观察到BMAL1表达和原合成基因之间存在显著的相关性.
抑制BMAL1导致细胞迁移的差异性增加以及肌肉细胞和LM细胞的入侵.
BMAL1 在人体子宫和LM内对原蛋白的产生起着至关重要的作用.

结论:

BMAL1参与人体肌肉膜和肌肉膜中的原蛋白生产,为子宫生理学和病理学提供了洞察力.
在原蛋白合成中BMAL1的作用强调了它在调节生殖生理学的重要性.
BMAL1成为抗白细胞瘤治疗的潜在治疗点.

关键词:

在BMAL1中,循环节的时钟是循环节的时间.原蛋白的合成在Leiomyoma和Leiomyoma之间.这种表达方式是Myometrium.

更多相关视频

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

Published on: October 12, 2013

Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta

Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta

Published on: February 7, 2018

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Role of Matrix Metalloproteases in Degradation of ECM

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...

Fibril-associated Collagen

Fibril-associated Collagen

Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...

Structural Protein Function

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...

Formation of Muscle Fibers from Myoblasts

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...