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在体外生物可用性,生物可访问性和抗氧化能力的差异取决于氨酸4'-O-葡萄糖酸的结合类型
Young Sung Jung1, Davin Jang1, Mi-Seon Kim1
1Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Food research international (Ottawa, Ont.)
|February 19, 2025
概括
葡萄糖化会影响卢托林的抗氧化作用. 素4'-O-alpha-glucoside (L4αG) 通过激活细胞通路,增强了抗氧化能力,而不仅仅是清除自由基.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 营养科学 营养科学
背景情况:
- 黄类化合物,如黄素,具有抗氧化特性.
- 糖基化可以改变黄酸的生物可用性和生物活性.
- 了解异构体特异性作用对于优化抗氧化功能至关重要.
研究的目的:
- 为了研究葡萄糖异常 (α与β) 对黄素抗氧化能力的影响.
- 为了比较体外4'-O-alpha-glucoside (L4αG) 和lutein 4'-O-beta-glucoside (L4βG) 的生物可访问性,生物可用性和生物活性.
- 阐明糖化对醇细胞内抗氧化作用的影响机制.
主要方法:
- 在体外分析生物可访问性,消化系统稳定性和甘释放.
- 使用H2O2诱导的Caco-2细胞进行细胞抗氧化活性测定.
- 研究信号通路,包括基激活蛋白激酶 (MAPK) 和核因子红色素-2-相关因子 (Nrf2).
主要成果:
- 与L4βG相比,L4αG显示出较低的水溶性,消化稳定性和糖释放率.
- 在Caco-2细胞中,L4αG显著缓解了细胞内氧化应激.
- 通过MAPK和Nrf2通路激活细胞抗氧化酶,L4αG增强了抗氧化能力.
结论:
- 在L4αG中的葡萄糖的α-解体能比L4βG更有效地增强细胞内抗氧化能力.
- L4αG的抗氧化作用主要通过激活内源抗氧化系统来调节.
- 这项研究提出了一种新的策略,用于开发基于高细胞亲和度的黄酸甘的天然抗氧化剂.
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