在几内亚猪中对连续传递的苏丹病毒进行深度测序,发现了适应性突变
Karla Emeterio1,2, Jonathan Audet1, Wenjun Zhu1
1Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.
Heliyon
|February 19, 2025
概括
苏丹病毒 (SUDV) 在几内亚猪中的适应揭示了病毒蛋白的关键突变,特别是糖蛋白和VP40. 这项研究确定了适应性热点,有助于了解SUDV的病原性和制定对策.
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 病原体适应 病原体适应
背景情况:
- 包括苏丹病毒 (SUDV) 在内的orthoebolavirus是Filoviridae家族中的高度致病性非细分负感RNA病毒.
- 东非的SUDV疫情具有很高的病例死亡率 (约. 50%),目前没有许可的治疗或预防药物.
- 适应小动物模型的filoviruses需要串行传递,这可以诱导影响病毒性的基因组变化.
研究的目的:
- 为了研究SUDV在几内亚猪的连续传递过程中获得的基因组突变.
- 确定SUDV基因组中的适应性热点,这些热点有助于致病性.
主要方法:
- 利用下一代测序来分析几内亚猪连续传递后的病毒基因组.
- 量化突变频率以确定适应性变化.
主要成果:
- 在适应后的高频率中确定了7个非同义和9个同义突变.
- 在葡萄糖蛋白和VP40中观察到大量的替代,在VP35和VP24中观察到单个非同义突变.
- 在VP40中发现了一种新的突变,与之前的桑格测序一致,但覆盖范围有所改善.
结论:
- 在几内亚猪中SUDV的连续传递导致了特定的适应性突变.
- 鉴定到的突变,特别是在糖蛋白和VP40中,可能是SUDV病原性的关键决定因素.
- 这项研究提供了对病毒适应和治疗开发的潜在点的见解.
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