Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Observational Studies01:11

Observational Studies

Observational studies are a type of analytical study where researchers observe events without any interventions. In other words, the researcher does not influence the response variable or the experiment's outcome.
There are three types of observational studies – Prospective, retrospective, and cross-sectional.
Prospective Study
Prospective studies, also known as longitudinal or cohort studies, are carried out by collecting future data from groups sharing similar characteristics. One example of...
Methods of Documentation VII: EMR01:30

Methods of Documentation VII: EMR

Electronic Medical Records (EMRs) primarily center around electronically documenting patients' health information within a single healthcare organization or practice. They contain essential clinical data related to a patient's medical history, diagnoses, medications, treatment plans, lab results, and other pertinent information relevant to the specific encounter or episode of care. EMRs are designed to streamline documentation and workflow processes within individual healthcare settings,...
Documentation in Long-Term and Home Healthcare Setting01:29

Documentation in Long-Term and Home Healthcare Setting

Documentation in long-term care facilities and home healthcare settings is crucial for ensuring continuous, coordinated, and comprehensive care for patients. Each setting has its specific documentation processes and tools:
Long-Term Care Facilities
Introduction to Epidemiology01:26

Introduction to Epidemiology

Epidemiology, known as the cornerstone of public health, involves studying the distribution and determinants of health-related events in defined populations and applying these insights to control health issues. This is essential for understanding how diseases spread, identifying populations at greater risk, and implementing measures to control or prevent outbreaks. Epidemiology addresses not only infectious diseases but also non-communicable conditions like cancer and cardiovascular disease,...
Pulmonary Embolism II: Diagnostic Studies and Interprofessional Care01:29

Pulmonary Embolism II: Diagnostic Studies and Interprofessional Care

Diagnosing Pulmonary EmbolismDiagnosing pulmonary embolism (PE) involves clinical assessment and advanced imaging tests. The preferred diagnostic tool is the spiral (helical) CT scan or CT angiography (CTA), which uses intravenous contrast media to visualize the pulmonary vasculature and identify emboli.A ventilation-perfusion (V/Q) scan is an alternative for patients unable to receive contrast media. This scan includes both perfusion and ventilation scanning. Perfusion scanning involves...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Inducible immortalized Dendritic Cells enable antigen-specific antibody production in a murine in vitro Immunization model.

PloS one·2026
Same author

Cross-variant immune shield: computational multiepitope vaccine design against B.617.2 to Omicron sub-lineages in SARS-CoV-2.

Journal of biomolecular structure & dynamics·2025
Same author

<i>AURKAPS1</i>, <i>HERC2P2</i> and <i>SDHAP1</i> pseudogenes: molecular role in development and progression of head and neck squamous cell carcinomas and their diagnostic utility.

Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation Oncology·2025
Same author

Automated determination of 8-OHdG in cells and tissue via immunofluorescence using a specially created antibody.

Biotechnology reports (Amsterdam, Netherlands)·2024
Same author

Analysis of antibodies from whole-cell immunization by a tANCHOR cell-based ELISA.

microPublication biology·2024
Same author

tANCHOR fast and cost-effective cell-based immunization approach with focus on the receptor-binding domain of SARS-CoV-2.

Biology methods & protocols·2023

相关实验视频

Updated: May 30, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

33.9K

为菌体显示库开发一个优化的基于细胞的选择系统.

Malgorzata Czarnecka1, Nicole Findik2, Anja Schlör2

  • 1Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, D-14476 Potsdam, Germany.

Biology methods & protocols
|February 19, 2025
PubMed
概括

这项研究引入了一种使用工程细胞系发现针对膜蛋白的抗体的新方法. 这种方法改善了抗原呈现,通过菌体显示查来更有效地识别抗体.

科学领域:

  • 生物技术是生物技术.
  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学

背景情况:

  • 体显示抗体的发现受到抗原呈现的挑战,特别是复杂的膜蛋白.
  • 使用全细胞的传统方法导致抗原密度低,背景噪声高.
  • 难以将膜蛋白呈现为它们的原生形状,阻碍了抗体的发展.

研究的目的:

  • 开发一种改进的方法,以在它们的原始状态下隔离对膜蛋白的抗体.
  • 建立一个高通量查平台,使用工程细胞系发现抗体.
  • 为了克服传统抗原呈现方法在菌体显示中的局限性.

主要方法:

  • 使用稳定转染的细胞系表达由EGFP调节的目标膜抗原 (EpCAM,NP65).
  • 采用高通量流动细胞计量用于选菌体显示库.
  • 优化了使用多克隆菌体池的查工作流程,并对选定的抗体进行了表征.

主要成果:

  • 成功建立了在细胞表面高密度呈现膜蛋白的系统.
  • 从一个纯粹的库中识别了特定于EpCAM的单链变量片段 (scFvs).
  • 证明了对复合表达的EpCAM抗体的特定结合.
关键词:
发现抗体的发现.膜蛋白质是一种膜蛋白质.菌体显示器可以显示菌体.再组合的人类抗体.选择的选择选择的选择.

更多相关视频

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

18.5K
Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

3.0K

相关实验视频

Last Updated: May 30, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

33.9K
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

18.5K
Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

3.0K

结论:

  • 开发的基于细胞的系统为针对本地膜蛋白的抗体发现提供了一个有效的平台.
  • 这种方法增强了抗原呈现,使得能够有效地隔离特定抗体.
  • 为产生针对具有挑战性的膜蛋白抗原的抗体提供了强大的策略.