与荒野城市界面 (WUI) 相关的表观遗传修饰 消防
Jaclyn M Goodrich1, Melissa A Furlong2, Derek J Urwin3,4
1Department of Environmental Health Sciences, University of Michigan School of Public Health, Ann Arbor, Michigan, USA.
Environmental and molecular mutagenesis
|February 19, 2025
概括
野外城市界面 (WUI) 火灾暴露改变了消防员的微RNA (miRNA) 表达,但没有显著改变DNA甲基化. 这表明与WUI消防危险相关的潜在表观遗传变化.
科学领域:
- 环境健康 环境健康
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 职业健康 职业健康 职业健康
背景情况:
- 野外城市界面 (WUI) 消防员面临着燃烧的植被和结构的显著有毒暴露,通常没有足够的呼吸保护.
- 已知表观遗传修饰,如microRNA (miRNA) 表达和DNA甲基化,会对有毒物质暴露作出反应,并与疾病发展有关.
- WUI火灾暴露对消防员表观遗传标记物的影响以前没有被研究过.
研究的目的:
- 调查WUI火灾暴露对消防员miRNA表达和DNA甲基化的影响.
- 为了确定与WUI火灾响应活动相关的特定表观遗传变化.
主要方法:
- 血液样本从99名消防员 (79名WUI暴露于火灾) 在基线和~10个月后收集.
- 使用nCounter人类v3miRNA表达面板,量化了800个miRNA的相对丰度.
- 通过使用Infinium EPIC阵列评估全基因组白细胞DNA甲基化.
- 线性混合模型被用来分析miRNA表达和DNA甲基化随时间变化的变化,调整混因素.
主要成果:
- 在所有消防员的基线和随访期间,观察到65个miRNAs表达的显著变化.
- 当局限于WUI火灾暴露的消防员时,50个miRNA显示出显著的变化.
- 瘤抑制剂miRNA hsa-miR-518c-3p的表达与WUI火响应显著相关.
- 在研究期间,没有发现DNA甲基化在统计学上显著的变化.
结论:
- 在野火季节的野地-城市界面火灾暴露可以改变消防员的miRNA表达.
- 在观察期间,DNA甲基化水平对WUI火灾暴露的反应没有显著变化.
- 研究结果表明,miRNA表达可能作为WUI火灾暴露和相关健康风险的敏感生物标志物.
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