基于HPLC指纹与多指标内容确定结合的Er Miao San的质量评估研究
Shuyan Xue1, Feichao Song2, Na Wei3
1The Third Clinical College, Shanxi University of Chinese Medicine, Taiyuan, China.
Biomedical chromatography : BMC
|February 19, 2025
概括
这项研究使用HPLC建立了Er Miao San (EMS) 的质量控制指纹,识别了类风湿性关节炎治疗的关键化合物和途径. 该方法有效地区分EMS批量,有助于质量评估和治疗理解.
科学领域:
- 草药质量控制 草药质量控制
- 传统中国医学 (TCM) 的分析分析.
- 药理学研究药理学研究
背景情况:
- 埃尔米桑 (EMS) 是一种传统的中医药,具有治疗类风湿性关节炎的潜力.
- 标准化的质量评估对于确保像EMS这样的草药的有效性和安全性至关重要.
- 了解EMS在类风湿性关节炎中的分子机制对于其临床应用至关重要.
研究的目的:
- 为Er Miao San (EMS) 建立一个高性能液态染色学 (HPLC) 指纹档案.
- 确定质量标记,以区分EMS批量,并评估样品质量.
- 通过网络药理学探索EMS在类风湿性关节炎治疗中的潜在治疗点和途径.
主要方法:
- 使用Agilent C18列的HPLC分析用于确定EMS指纹.
- 集群分析和主要组件分析 (PCA) 使用SPSS和SIMCA软件进行.
- 使用包括STRING和Cytoscape在内的网络药理技术来确定目标和途径,随后进行GO和KEGG丰富分析.
主要成果:
- 通过HPLC成功建立了EMS的指纹档案,包括15个常见的峰值,其中9个被确定.
- 根据其化学特征,十批EMS被分为三个不同的集群.
- 通过网络药理学分析确定了四个指数差异组件,并透露了228个交叉目标.
结论:
- 开发的HPLC方法提供了一种有效的手段来分析来自不同来源的EMS质量变化.
- 确定的目标和途径为治疗类风湿性关节炎的各种EMS批次的差异性影响提供了重要的见解.
- 这项研究有助于EMS在类风湿性关节炎治疗中的标准化和合理利用.
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