在低气条件下,LNC159c通过miR159c在Malus spectabilis中负面调节安托西亚尼生物合成
Jiaxin Meng1, Han Wang2, Yixin Liu2
1Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Plant, cell & environment
|February 19, 2025
概括
低的条件降低了长非编码RNA (lncRNA) LNC159c,这通常限制了miR159c. 这种减少释放了对MsMYB10的抑制,从而增强了植物中的安托氨酸生物合成.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因调节 基因调节
- 生物化学 生化学
背景情况:
- 低的条件往往会诱导植物中的安东素生产.
- 之前的研究集中在转录因子和结构基因上,对这种途径中长非编码RNA (lncRNA) 的理解有限.
- lncRNAs因其在基因表达调节中的作用越来越受认可.
研究的目的:
- 为了研究 lncRNAs 在低条件下调节氨酸生物合成中的作用.
- 为了识别参与这个过程的特定 lncRNA.
- 阐明 lncRNAs 控制抗生素积累的分子机制.
主要方法:
- 在低应激下识别和表达lncRNAs的分析.
- 双露西法酶记者测定以确认miRNA-目标相互作用.
- 酵母单杂交和双化酶测定以验证转录因子结合.
- 稳定和短暂的过度表达/沉默实验在果和M. spectabilis.
主要成果:
- 在低条件下,LNC159c表达被降低了.
- LNC159c作为miR159c的宿主基因,其过度表达增加了miR159c水平.
- 发现miR159c可以抑制MsMYB10的表达.
- MsMYB10直接与F3'H的促进体结合,F3'H是安托氨酸生物合成中的关键酶.
- 过度表达LNC159c抑制了安托氨酸生物合成,而MsMYB10过度表达促进了它.
结论:
- 在低的条件下,减少LNC159c导致减少miR159c,缓解MsMYB10的抑制.
- 这一途径最终增强了安东素的积累.
- lncRNAs,以LNC159c为例,在植物对营养可用性的反应中发挥着重要的调节作用,特别影响了氨酸生物合成.
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