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与UDPG再生相结合的UDP-Glycosyltransferases工程促进了Mogroside V的高效转化

Man Zhao^1,2, Donglian Lai^1,2, Xiaoli Jia^1,2

¹Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China.

Journal of agricultural and food chemistry

|February 19, 2025

概括

研究人员设计了糖转移酶 (UGTs),以高效地合成Siraitia grosvenorii中的天然甜味剂摩格罗化物V. 这种新型的酶系统使得mogrol可以直接转化为mogroside V,从而提高了生产潜力.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
自然产品化学化学

背景情况:

摩格罗化物V是一种来自Siraitia grosvenorii的三烯甜味剂,通过选择性糖化摩格罗的合成.
工程甘氨基转移酶 (UGTs) 为改进的摩格罗化物V生物合成提供了一条途径.

研究的目的:

设计UGTs (UGTM1和UGTM2) 来增强mogrol的选择性糖化.
开发一个高效的体外酶系统用于mogroside V.生产.

主要方法:

用局部定向的突变生成来将UGTM1和UGTM2改造成UGTM1-3和UGTM2-4.
一个UDP-葡萄糖 (UDPG) 再生系统与工程UGT相结合.
通过使用UGTM1-3,UGTM2-4和AtSUS1建立了体外多酶级联系统.

主要成果:

改造后的UGTM1-3和UGTM2-4分别显示酶活性增加了2.88倍和3.60倍.
结合系统实现了从mogrol到mogroside V的直接转换率为18.2%,没有外源的UDPG.
通过使用工程酶和再生系统,证明了mogroside V的成功生物合成.

结论:

工程UGT为mogroside V生物合成提供了一个强大的平台.
开发的"体外级联系统"为生产mogroside V.提供了一种高效且潜在可扩展的方法.
这项研究推进了对有价值的自然产品的酶合成策略.

关键词:

阿拉比多普西斯的糖糖合成酶 (AtSUS1)在UDPG的再生过程中,工程工程工程工程工程葡萄糖转移酶的使用方法摩格罗塞德V 在线观看

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