用Corynebacterium glutamicum的代谢工程来生产不同类型的三类植物
Jingzhi Li1, Xinxin Wang1, Xahnaz Xokat1
1Key Laboratory of Medical Molecule Science and Pharmaceutical Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.
ACS synthetic biology
|February 19, 2025
概括
这项研究对Corynebacterium glutamicum进行了改造,使其能够高效地产生三类. 研究人员增强了烯合成,并引入了新的途径,使得像α-amyrin和ursolic acid这样有价值的化合物的产生成为可能.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 三聚类是具有多种生物活动的天然化合物.
- 在Corynebacterium glutamicum中,三类生物合成是有限的,阻碍了生产.
- 目前的研究主要集中在C. glutamicum中的烯生产上.
研究的目的:
- 开发Corynebacterium glutamicum作为一个用于三类生物合成的多功能平台.
- 为了改造C. glutamicum以提高烯及其衍生物的生产.
- 建立C. glutamicum中各种三类化合物的新合成途径.
主要方法:
- 代谢工程策略和发酵优化以改善素标位.
- 来自Arabidopsis thaliana的斯夸伦环氧化酶的异质表达.
- 引入氧化二二烯环酶和P450酶用于下游三二烯化合物合成.
主要成果:
- 达到400.1 mg/L的烯标位,细胞内积累超过97%.
- 成功合成了201.9 mg/L的3S-2,3-oxidosqualene (SQO) 和264.9 mg/L的3S,22S-2,3,22,23-dioxidosqualene (SDO) 在201.9 mg/L.
- 在C. glutamicum中首次产生了α-amyrin (65.3 mg/L),α-onocerin (136.85 mg/L) 和ursolic acid (486 μg/L).
结论:
- 科里尼细菌 (Corynebacterium glutamicum) 作为一个有效的底盘,用于新的三烯酸生物合成.
- 这些工程路径使得从石烯中生产出多种不同的三类化合物.
- 这项工作为大规模生产高价值的三烯酸提供了一个强大的平台.
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